25-Hydroxyvitamin D3-1?-hydroxylase and vitamin D receptor gene expression in human colonic mucosa is elevated during early cancerogenesis

Heide S. Cross, Petra Bareis, Hofer Harald, Martin Georg Bischof, Erika Bajna, Stefan Kriwanek, Elisabeth Bonner, Meinrad Peterlik

Veröffentlichungen: Beitrag in FachzeitschriftMeeting Abstract/Conference Paper

Abstract

Human colorectal cancer cells not only express the nuclear vitamin D receptor (VDR) but are also endowed with 25-hydroxy-vitamin D3-1α-hydroxylase activity and therefore are able to produce the specific ligand for the VDR, the hormonally active steroid 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3). In the present study we show by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) as well as by Western blotting and immunohistochemical methods, that in human large intestinal carcinomas expression of the genes encoding the 25-(OH)D3-1α-hydroxylase as well as the VDR increases in parallel with ongoing dedifferentiation in the early phase of cancerogenesis, whereas in poorly differentiated late stage carcinomas only low levels of the respective mRNAs can be detected. This indicates that, through up-regulation of this intrinsic 1α,25(OH)2D3/VDR system which mediates the anti-mitotic effects of the steroid hormone, colorectal cancer cells are apparently able to increase their potential for an autocrine counter-regulatory response to neoplastic cell growth, particularly in the early stages of malignancy. Copyright Œ 2001 Elsevier Science Inc.
OriginalspracheEnglisch
Seiten (von - bis)287-292
Seitenumfang6
FachzeitschriftSteroids
Jahrgang66
Ausgabenummer3-5
DOIs
PublikationsstatusVeröffentlicht - 2001
Extern publiziertJa

ÖFOS 2012

  • 1030 Physik, Astronomie

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