TY - JOUR
T1 - A dynamic model of growth phase of bio-conversion of methane to polyhydroxybutyrate using dynamic flux balance analysis
AU - Nasershariat, Mohadeseh
AU - Pishvaie, Mahmoud Reza
AU - Boozarjomehry, Ramin Bozorgmehry
AU - Waldherr, Steffen
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
PY - 2024/4
Y1 - 2024/4
N2 - Biological conversion of waste methane to biodegradable plastics is a way of reducing their production cost. This study addresses the computational modeling of the growth phase reactor of the process of polyhydroxybutyrate production. The model was used for investigating the effect of gas recycling and inlet gas retention time on the reactor performance. The model was run by the use of a genome-scale metabolic network of Methylocystis hirsuta in a dynamic flux balance analysis framework. The reactor has been modeled for two separate feeding scenarios: a pure methane feed and a biogas feed. The mass transfer coefficient parameter was predicted as a function of superficial gas velocities by the regression of data from published experiments. The results show an increase of removal efficiency by 38% and biomass concentration by 2.8 g/L with the increase of gas recycle ratio from 0 to 30 at the empty bed residence time of 60 min.
AB - Biological conversion of waste methane to biodegradable plastics is a way of reducing their production cost. This study addresses the computational modeling of the growth phase reactor of the process of polyhydroxybutyrate production. The model was used for investigating the effect of gas recycling and inlet gas retention time on the reactor performance. The model was run by the use of a genome-scale metabolic network of Methylocystis hirsuta in a dynamic flux balance analysis framework. The reactor has been modeled for two separate feeding scenarios: a pure methane feed and a biogas feed. The mass transfer coefficient parameter was predicted as a function of superficial gas velocities by the regression of data from published experiments. The results show an increase of removal efficiency by 38% and biomass concentration by 2.8 g/L with the increase of gas recycle ratio from 0 to 30 at the empty bed residence time of 60 min.
KW - Dynamic flux balance analysis
KW - Genome-scale metabolic network
KW - Methane
KW - Methylocystis hirsuta
KW - Polyhydroxybutyrate
UR - http://www.scopus.com/inward/record.url?scp=85187947676&partnerID=8YFLogxK
U2 - 10.1007/s00449-024-02966-w
DO - 10.1007/s00449-024-02966-w
M3 - Article
C2 - 38492006
AN - SCOPUS:85187947676
SN - 1615-7591
VL - 47
SP - 463
EP - 474
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
IS - 4
ER -