Accurate LC-ESI-MS/MS quantification of 2'-deoxymugineic acid in soil and root related samples employing porous graphitic carbon as stationary phase and a 13C 4-labeled internal standard

Yvonne Schindlegger, Eva Oburger, Barbara Gruber, Walter D. C. Schenkeveld, Stephan Krämer, Markus Puschenreiter, Gunda Koellensperger, Stephan Hann (Korresp. Autor*in)

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

For the first time the phytosiderophore 2'-deoxymugineic acid (DMA) could be accurately quantified by LC-MS/MS in plant and soil related samples. For this purpose a novel chromatographic method employing porous graphitic carbon as stationary phase combined with ESI-MS/MS detection in selected reaction monitoring was developed. Isotope dilution was implemented by using in-house synthesized DMA as external calibrant and 13C 4-labeled DMA as internal standard (concentration levels of standards 0.1-80 μM, determination coefficient of linear regression R 2 > 0.9995). Sample preparation involved acidification of the samples in order to obtain complete dissociation of metal-DMA complexes. Excellent matrix related LOD and LOQ depending on different experimental setups were obtained in the range of 3-34 nM and 11-113 nM, respectively. Standard addition experiments and the implementation of the internal 13C 4-DMA standard proved the accuracy of the quantification strategy even in complex matrices such as soil solution. The repeatability of the method, including sample preparation, expressed as short- and long term precision was below 4 and 5% RSD, respectively. Finally, application in the context of plant and soil research to samples from rhizosphere sampling via micro suction cups, from soil solutions and soil adsorption/extraction studies revealed a DMA concentration range from 0.1 to 235 μM.

OriginalspracheEnglisch
Seiten (von - bis)1375-1385
Seitenumfang11
FachzeitschriftElectrophoresis
Jahrgang35
Ausgabenummer9
DOIs
PublikationsstatusVeröffentlicht - Mai 2014

ÖFOS 2012

  • 104004 Chemische Biologie
  • 104023 Umweltchemie
  • 104002 Analytische Chemie
  • 105906 Umweltgeowissenschaften

Zitationsweisen