Alternative approaches for the estimation of the band broadening parameters in single-detection size exclusion chromatography

Jorge R. Vega, Irene Schnöll-Bitai

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed


New approaches for the determination of the extent of symmetric and asymmetric band broadening (BB) in size exclusion chromatography (SEC) are presented. For this purpose raw data was simulated by starting with either a theoretical Poisson number chain length distribution (NCLD), or a log-normal weight chain length distribution (WCLD). Each distribution was first converted to a BB-free mass chromatogram, as typically obtained from a standard differential refractive index detector. Then, the broadened (or "measured") chromatograms were simulated by convoluting the BB-free chromatograms with a BB function, which was assumed to follow symmetrical (Gauss) as well as unsymmetrical (exponentially modified Gauss) function. A broad range of BB parameters (standard deviation, sBB, and exponential decay, tBB) was used for the simulations. The approaches are based on the determination of the points of inflection belonging to the peak of the broadened chromatogram, and closed as well as empirically derived equations connecting the peak width, its variance, and the parameters sBB and tBB. The developed methods are applicable for Poisson distributions well above a peak chain length of 100. Π2005 Elsevier B.V. All rights reserved.
Seiten (von - bis)102-112
FachzeitschriftJournal of Chromatography A
PublikationsstatusVeröffentlicht - 2005

ÖFOS 2012

  • 104017 Physikalische Chemie