Biotransformations monitored in situ by proton nuclear magnetic resonance spectroscopy

Lothar Brecker, Douglas W. Ribbons (Korresp. Autor*in)

Veröffentlichungen: Beitrag in FachzeitschriftReviewPeer Reviewed

Abstract

One-dimensional Fourier-transform proton nuclear magnetic resonance (1H-NMR) spectroscopy can be used to study biotransformations in situ, in vivo and in aqua (1H2O). Although an insensitive method, it rapidly provides solution-structural information of mixtures of diverse compounds that are used and formed during enzymic reactions and culture fermentations; the samples do not require any physical or chemical processing for analysis. The absolute stereochemistry of some reactions can also be determined, and assessments of metabolic fluxes made. This technique, with appropriate modifications, is of obvious value for on-line assessments of industrial fermentation processes. Copyright (C) 2000 Elsevier Science Ltd.
OriginalspracheEnglisch
Seiten (von - bis)197-202
Seitenumfang6
FachzeitschriftTrends in Biotechnology
Jahrgang18
Ausgabenummer5
DOIs
PublikationsstatusVeröffentlicht - 2000

ÖFOS 2012

  • 1040 Chemie

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