TY - JOUR
T1 - Copper bioreduction and nanoparticle synthesis by an enrichment culture from a former copper mine
AU - Kimber, Richard L
AU - Elizondo, Gretta
AU - Jedyka, Klaudia
AU - Boothman, Christopher
AU - Cai, Rongsheng
AU - Bagshaw, Heath
AU - Haigh, Sarah J
AU - Coker, Victoria S
AU - Lloyd, Jonathan R
N1 - Publisher Copyright:
© 2023 The Authors. Environmental Microbiology published by Applied Microbiology International and John Wiley & Sons Ltd.
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Microorganisms can facilitate the reduction of Cu2+ , altering its speciation and mobility in environmental systems and producing Cu-based nanoparticles with useful catalytic properties. However, only a few model organisms have been studied in relation to Cu2+ bioreduction and little work has been carried out on microbes from Cu-contaminated environments. This study aimed to enrich for Cu-resistant microbes from a Cu-contaminated soil and explore their potential to facilitate Cu2+ reduction and biomineralisation from solution. We show that an enrichment grown in a Cu-amended medium, dominated by species closely related to Geothrix fermentans, Azospira restricta and Cellulomonas oligotrophica, can reduce Cu2+ with subsequent precipitation of Cu nanoparticles. Characterisation of the nanoparticles with (scanning) transmission electron microscopy, energy-dispersive x-ray spectroscopy and electron energy loss spectroscopy supports the presence of both metallic Cu(0) and S-rich Cu(I) nanoparticles. This study provides new insights into the diversity of microorganisms capable of facilitating copper reduction and highlights the potential for the formation of distinct nanoparticle phases resulting from bioreduction or biomineralisation reactions. The implications of these findings for the biogeochemical cycling of copper and the potential biotechnological synthesis of commercially useful copper nanoparticles are discussed.
AB - Microorganisms can facilitate the reduction of Cu2+ , altering its speciation and mobility in environmental systems and producing Cu-based nanoparticles with useful catalytic properties. However, only a few model organisms have been studied in relation to Cu2+ bioreduction and little work has been carried out on microbes from Cu-contaminated environments. This study aimed to enrich for Cu-resistant microbes from a Cu-contaminated soil and explore their potential to facilitate Cu2+ reduction and biomineralisation from solution. We show that an enrichment grown in a Cu-amended medium, dominated by species closely related to Geothrix fermentans, Azospira restricta and Cellulomonas oligotrophica, can reduce Cu2+ with subsequent precipitation of Cu nanoparticles. Characterisation of the nanoparticles with (scanning) transmission electron microscopy, energy-dispersive x-ray spectroscopy and electron energy loss spectroscopy supports the presence of both metallic Cu(0) and S-rich Cu(I) nanoparticles. This study provides new insights into the diversity of microorganisms capable of facilitating copper reduction and highlights the potential for the formation of distinct nanoparticle phases resulting from bioreduction or biomineralisation reactions. The implications of these findings for the biogeochemical cycling of copper and the potential biotechnological synthesis of commercially useful copper nanoparticles are discussed.
KW - Copper
KW - Nanoparticles/chemistry
UR - http://www.scopus.com/inward/record.url?scp=85170697806&partnerID=8YFLogxK
U2 - 10.1111/1462-2920.16488
DO - 10.1111/1462-2920.16488
M3 - Article
C2 - 37697680
SN - 1462-2912
VL - 25
SP - 3139
EP - 3150
JO - Environmental Microbiology
JF - Environmental Microbiology
IS - 12
ER -