Determination of Vitamin K in Milk and Infant Formulas by Liquid Chromatography: Collaborative Study

Harvey E. Indyk, David C. Woollard, Millie Barber, Carrie Berge, Sarah Booth, Malcolm Burn, Adriano Cecco, Scott Christiansen, Kathy Cook, Ursula Coors, Francesca Dapra, Kenneth Davidson, Marion P. De Roode, Natalie Dumais, Michael Edwards, Chris Ellis, Ibrahim Elmadfa, Giuliana Fedrigo, Bertram Fong, Pamela GillilandSusan Hannaford, Peter Hofmann, Peter Horstmann, Marija Jagodic, Maurice Kerr, Kaname Kodaka, Terhi Koivu-Tikkanen, Erik Konings, Jim Kyval, Bernard Lacheron, Paul Lawrence, Emile Ledoyen, Pamela Manzi, Hisashi Matsuda, Adriana McCamley, Donald Myers, Janet Nelson, Rachel Noble, Lisa Oehrl, Gianfranco Panfili, Richard Pelletier, Vieno Piironen, Laura Pizzoferrato, Sal Regala, Koichi Saito, Karen Schimpf, Willy Schuep, Leon Schurgers, Henry Simons, Enrico Tagliaferri, Henk Thijssen, Christian Tremblay, Marie Jose Trisconi, Cees Vermeer, Wijnand Verschoor, Pat Vyas, Karl Heinz Wagner, Gerald Woollard

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed


A simple procedure for determination of vitamin Ki was developed for routine compliance monitoring of supplemented infant formula and measurement of endogenous levels in milk and milk powders. Samples are digested with lipase and extracted into hexane; an aliquot is evaporated, reconstituted in methanol, and analyzed by reversed-phase LC. Post-column zinc reduction of phylloquinone facilitates detection by fluorescence. The procedure was subjected to an AOAC collaborative study involving 8 materials, each in blind duplicate, across the range of 5-120 μg/100 g solids and including NIST 1846 reference material. Thirty-three laboratories returned valid data which were then statistically analyzed for outliers and precision parameters. Mean RSDR (%) was 6.53 (4.33-10.94), with a mean HORRAT value of 0.33 (0.23-0.43) and RSDr:RSDR ratio of 0.74. K1 isomers (cis and trans) were aggregated with conventional C18 columns, but may be selectively estimated with use of the C30 column.

Seiten (von - bis)121-130
FachzeitschriftJournal of AOAC International
PublikationsstatusVeröffentlicht - 2000

ÖFOS 2012

  • 303009 Ernährungswissenschaften