Direct high-performance liquid chromatographic enantioseparation of secondary amino acids on Cinchona alkaloid-based chiral zwitterionic stationary phases. Unusual temperature behavior

Istvan Ilisz, Zsanett Gecse, Zoltan Pataj, Ferenc Fulop, Geza Toth, Wolfgang Lindner, Antal Peter (Korresp. Autor*in)

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

Two chiral stationary phases containing a quinine- or a quinidine-based zwitterionic ion-exchanger as chiral selector were applied for the enantioseparation of 27 unusual cyclic secondary α-amino acids. The effects of the nature and concentration of the bulk solvent, the acid and base additives, the structures of the analytes and temperature on the enantioresolution were investigated. To study the effects of temperature and to obtain thermodynamic parameters, experiments were carried out at constant mobile phase compositions in the temperature range -5 to 55. °C. The thermodynamic parameters indicated that in most cases the separations were enthalpy-driven, but some entropy-driven separations were also observed. The sequence of elution of the enantiomers was determined in most cases.

OriginalspracheEnglisch
Seiten (von - bis)169-177
Seitenumfang9
FachzeitschriftJournal of Chromatography A
Jahrgang1363
DOIs
PublikationsstatusVeröffentlicht - 10 Okt. 2014

ÖFOS 2012

  • 104002 Analytische Chemie

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