Diversity of reed (Phragmites Australis) stem biofilm bacterial communities in two Hungarian soda lakes

Anna Rusznyák, Gitta Szabó, Beatrix Pollák, Viktória Vágány, M. Palatinszky

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

From reed biofilm samples of Kelemen-szék (Kiskunsg National Park, KNP) and Nagy-Vadas (Hortobágy National Park, HNP) altogether 260 bacterial isolates were gained after serial dilutions and plating onto different media. Following a primary selection 164 strains were investigated by "traditional" phenotypic tests and clustered by numerical analysis. Fifty-six representative strains were selected to ARDRA and 16S rDNA sequence analysis for identification. Strains were identified as members of genera Agrobacterium, Paracoccus, Halomonas, Pseudomonas, Bacillus, Planococcus and Nesterenkonia. The species diversity was also investigated by a cultivation independent method. A clone library was constructed using the community DNA isolated from the biofilm sample of Kelemen-szék. Screening of the 140 bacterial clones resulted in 45 different ARDRA groups. Sequence analysis of the representatives revealed a great phylogenetic diversity. A considerable majority of the clones was affiliated with uncultured bacterial clones (with sequence similarity between 93 and 99%) originating from diverse environmental samples (for example salt marshes, compost or wastewater treatment plants). The DNA sequences of other clones showed the presence of genera Flavobacterium, Sphingobacterium, Pseudomonas and Agrobacterium.

OriginalspracheEnglisch
Seiten (von - bis)339-352
Seitenumfang14
FachzeitschriftActa Microbiologica et Immunologica Hungarica
Jahrgang54
Ausgabenummer4
DOIs
PublikationsstatusVeröffentlicht - Dez. 2007
Extern publiziertJa

ÖFOS 2012

  • 106022 Mikrobiologie
  • 106003 Biodiversitätsforschung

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