DNA binding and antiproliferative activity toward human carcinoma cells of copper(ii) and zinc(ii) complexes of a 2,5-diphenyl[1,3,4]oxadiazole derivative

Alessio Terenzi, Mirco Fanelli, Gianluca Ambrosi, Stefano Amatori, Vieri Fusi, Luca Giorgi, Vincenzo Turco Liveri, Giampaolo Barone

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

The interaction of calf thymus DNA with [CuL(ClO 4)]ClO 4·H 2O (1) and [ZnLBr]Br·H 2O (2) (L = 9,12,15,18,27,28-hexaaza-29-oxatetracyclo[24.2.1.0 2,7.0 20,25]enneicosa-2,4,6,20,22,24,26,28 1-octaene) dicationic complexes in aqueous solution at neutral pH, was investigated by variable-temperature UV-vis absorption, circular dichroism and fluorescence spectroscopy. The values of the DNA-binding constants of these complexes, determined by competitive binding spectrofluorimetric titrations of ethidium bromide (EB)-DNA solutions, are (6.7 ± 0.5) × 10 6 M -1 for CuL 2+ and (4.7 ± 0.5) × 10 5 M -1 for ZnL 2+. These data together with a through analysis of the spectroscopic behaviour consistently suggest that both compounds are effective DNA binders. Interestingly, the DNA-binding strength of these complexes has been found to be correlated to their in vitro cytotoxic activity toward human breast carcinoma cells, although the complex with lower DNA-binding affinity is more active. In fact, biological studies showed that when the compounds are delivered through the cell membrane by a lipidic carrier, the cell survival is sensibly reduced, up to 58% with 1 and to 31% with 2.

OriginalspracheEnglisch
Seiten (von - bis)4389-4395
Seitenumfang7
FachzeitschriftDalton Transactions
Jahrgang41
Ausgabenummer15
DOIs
PublikationsstatusVeröffentlicht - 21 Apr. 2012
Extern publiziertJa

ÖFOS 2012

  • 104003 Anorganische Chemie

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