Abstract
Glucose variability (GV) is a phenomenon that describes fluctuations in blood glucose levels over the course of a day. As part of the investigation of risk factors for coronary heart disease, GV is increasingly becoming the focus of scientific attention. Scattering GV can contribute to the development of metabolic syndrome and type 2 diabetes. Hyperglycemia can lead to oxidative stress, which results in molecular damage due to an accumulation of reactive oxygen species (ROS). To obtain more information on the immediate effects of GV, 10 healthy men aged 21–30 years were administered intravenous glucose continuously or as a repeated bolus over a 48-hour period in a crossover study design.Whole blood was analysed for DNA damage using the Comet assay using three different incubation solutions (lysis buffer, H2O2 and the lesion-specific enzyme formamidopyrimidine DNA glycosylase (FPG)) and plasma for various markers of oxidative stress (protein carbonyls (PC), unconjugated bilirubin (UCB) and total antioxidant potential (FRAP). A significant time effect was found for the three incubation solutions for DNA damage, PC and UCB. This can potentially be attributed to circadian changes. However, no differences were observed for any of the markers between the two intervention groups. In conclusion, it was shown that bolus glucose administration had no significant acute effect on DNA damage and oxidative stress markers in healthy men compared to continuous administration.
Originalsprache | Englisch |
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Titel | Abstracts of the 52nd EEMGS & 15th ICAW Meeting |
Untertitel | 52nd European Environmental Mutagenesis and Genomics Society (EEMGS) & 15th International Comet Assay Workshops (ICAW) meeting |
Redakteure*innen | Marko Geric, Goran Gajski |
Seiten | 117-117 |
Band | 75 |
ISBN (elektronisch) | 1848-6312 |
Publikationsstatus | Veröffentlicht - 2025 |
Veranstaltung | 52nd European Environmental Mutagenesis and Genomics Society (EEMGS) & 15th International Comet Assay Workshops (ICAW) meeting - Rovinj, Rovinj, Kroatien Dauer: 23 Sept. 2024 → 27 Sept. 2024 |
Konferenz
Konferenz | 52nd European Environmental Mutagenesis and Genomics Society (EEMGS) & 15th International Comet Assay Workshops (ICAW) meeting |
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Land/Gebiet | Kroatien |
Ort | Rovinj |
Zeitraum | 23/09/24 → 27/09/24 |
ÖFOS 2012
- 303009 Ernährungswissenschaften
- 301303 Medizinische Biochemie
- 301110 Physiologie