Effects of phospholipids on skin: use of primary human keratinocytes and fibroblasts as 2D cell culture models

Human primary keratinocytes and fibroblasts are classic 2D cell culture models that are highly useful for the assessment of the skin compatibility of cosmetic and pharmaceutical formulation compounds. The skin compatibility of phospholipid-based nanoemulsions was investigated using different cytotoxicity assays. Phospholipids are known to be versatile surface-active agents that can be used for the production of a range of cosmetic or pharmaceutical formulations. Evaluation of biocompatibility using cell culture systems is a vital step when developing new formulations. A wide variety of established cell lines are available for research purposes. Skin used for the isolation of cells stems from abdomen, back, or breast of surplus skin. A more recent approach refrains from using feeder cells, but instead relies on serum-free media that are low in calcium. The chapter investigates the skin compatibility of individual additives or to evaluate new dermal formulations.