Abstract
Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomatosensitized
patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin
(Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher
than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional
protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were
purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying
IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology
between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins
Originalsprache | Englisch |
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Seiten (von - bis) | 1111-1122 |
Seitenumfang | 12 |
Fachzeitschrift | Journal of Proteome Research |
Jahrgang | 8 |
Ausgabenummer | 3 |
DOIs | |
Publikationsstatus | Veröffentlicht - 2009 |
ÖFOS 2012
- 106002 Biochemie
- 1030 Physik, Astronomie
- 106037 Proteomik