Evidence for novel tomato seed allergens: Ige-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling

Olivia Y. Bässler, Julia Weiss, Stefanie Wienkoop, Karola Lehmann, Christian Scheler, Sabine Dölle, Dietmar Schwarz, Philipp Franken, Eckhard George, Margitta Worm, Wolfram Weckwerth (Korresp. Autor*in)

    Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

    Abstract

    Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomatosensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins
    OriginalspracheEnglisch
    Seiten (von - bis)1111-1122
    Seitenumfang12
    FachzeitschriftJournal of Proteome Research
    Jahrgang8
    Ausgabenummer3
    DOIs
    PublikationsstatusVeröffentlicht - 2009

    ÖFOS 2012

    • 106002 Biochemie
    • 1030 Physik, Astronomie
    • 106037 Proteomik

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