TY - JOUR
T1 - Expression of the ubiquitin variant ubR48 decreases proteolytic activity in Arabidopsis and induces cell death
AU - Schlögelhofer, Peter
AU - Garzon, Marcus
AU - Kerzendorfer, Claudia-Anna
AU - Nizhynska, Victoria
AU - Bachmair, Andreas
N1 - DOI: 10.1007/s00425-005-0121-z
Coden: PLANA
Affiliations: Institute of Botany, University of Vienna, Rennweg 14, 1030 Vienna, Austria; Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, Carl-von-LinneŽ-Weg 10, 50829 Cologne, Germany; Department of Chromosome Biology, Vienna Biocenter 2, University of Vienna, Dr. Bohr-Gasse 1, 1030 Vienna, Austria; Center for Brain Research, Medical University of Vienna, Spitalgasse 4, 1090 Vienna, Austria
Adressen: Bachmair, A.; Department of Plant Developmental Biology; Max Planck Institute for Plant Breeding Research; Carl-von-LinneŽ-Weg 10 50829 Cologne, Germany; email: [email protected]
Source-File: MFPLUniWienScopus.csv
Import aus Scopus: 2-s2.0-33744953644
Importdatum: 07.12.2006 15:12:20
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
PY - 2006
Y1 - 2006
N2 - The ubiquitin-proteasome pathway is the major route for protein degradation in eukaryotes. We show here that this pathway can be inhibited in Arabidopsis thaliana by expression of a ubiquitin variant that contains Arg instead of Lys at position 48 (ubR48). A major consequence of ubR48 expression is the induction of cell death. Cell death induction coincides with the appearance of reactive oxygen intermediates, but is independent of salicylic acid. We found changes in expression of some defense-related genes, but these changes are apparently insufficient to cause alterations in the response to a bacterial pathogen. Expression of ubR48 from an inducible gene allowed investigation of kinetic parameters of cell death induction. In the absence of additional stress factors, slow death processes dominate if the transgene is induced in seedlings older than 2 weeks. The inducible gene also allowed isolation of suppressor mutants. Expression of ubR48 may cause changes similar to inhibition of the proteasome, which also induces various forms of cell death. Thus, ubR48 is a tool to manipulate protein turnover and to probe cell death programs in plants. Œ Springer-Verlag 2005.
AB - The ubiquitin-proteasome pathway is the major route for protein degradation in eukaryotes. We show here that this pathway can be inhibited in Arabidopsis thaliana by expression of a ubiquitin variant that contains Arg instead of Lys at position 48 (ubR48). A major consequence of ubR48 expression is the induction of cell death. Cell death induction coincides with the appearance of reactive oxygen intermediates, but is independent of salicylic acid. We found changes in expression of some defense-related genes, but these changes are apparently insufficient to cause alterations in the response to a bacterial pathogen. Expression of ubR48 from an inducible gene allowed investigation of kinetic parameters of cell death induction. In the absence of additional stress factors, slow death processes dominate if the transgene is induced in seedlings older than 2 weeks. The inducible gene also allowed isolation of suppressor mutants. Expression of ubR48 may cause changes similar to inhibition of the proteasome, which also induces various forms of cell death. Thus, ubR48 is a tool to manipulate protein turnover and to probe cell death programs in plants. Œ Springer-Verlag 2005.
U2 - 10.1007/s00425-005-0121-z
DO - 10.1007/s00425-005-0121-z
M3 - Article
SN - 0032-0935
VL - 223
SP - 684
EP - 697
JO - Planta: an international journal of plant biology
JF - Planta: an international journal of plant biology
IS - 4
ER -