FtsZ-mediated fission of a cuboid bacterial symbiont

Philipp M. Weber, Gabriela F. Paredes, Tobias Viehboeck, Nika Pende, Jean Marie Volland, Olivier Gros, Michael VanNieuwenhze, Jörg Ott, Silvia Bulgheresi (Korresp. Autor*in)

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

Less than a handful of cuboid and squared cells have been described in nature, which makes them a rarity. Here, we show how Candidatus Thiosymbion cuboideus, a cube-like gammaproteobacterium, reproduces on the surface of marine free-living nematodes. Immunostaining of symbiont cells with an anti-fimbriae antibody revealed that they are host-polarized, as these appendages exclusively localized at the host-proximal (animal-attached) pole. Moreover, by applying a fluorescently labeled metabolic probe to track new cell wall insertion in vivo, we observed that the host-attached pole started septation before the distal one. Similarly, Ca. T. cuboideus cells immunostained with an anti-FtsZ antibody revealed a proximal-to-distal localization pattern of this tubulin homolog. Although FtsZ has been shown to arrange into squares in synthetically remodeled cuboid cells, here we show that FtsZ may also mediate the division of naturally occurring ones. This implies that, even in natural settings, membrane roundness is not required for FtsZ function.
OriginalspracheEnglisch
Aufsatznummer103552
Seitenumfang14
FachzeitschriftIscience
Jahrgang25
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - 21 Jan. 2022

ÖFOS 2012

  • 106022 Mikrobiologie

Zitationsweisen