Fungi distinguish between dead litter and living plants using a class XIII GPCR responsible for posttranscriptional regulation of enzyme production

Monika Schmoll

Veröffentlichungen: Beitrag zu KonferenzSonstiger Konferenzbeitrag

Abstract

Fungal metabolism and enzyme production are regulated by nutrient availability and by interactions with the living environment. We investigated the mechanisms underpinning adaptation of the biotechnological fungus Trichoderma reesei to decaying plant biomass versus living plants. We found that concentration-gated response to glucose, the main molecule sensed from dead plant biomass, is mediated by a conserved signaling pathway downstream of G protein coupled receptors (GPCRs), while the carbon catabolite repressor CRE1 is critical for glucose concentration gating. The GPCRs CSG1 and CSG2 are further required for root colonization and formation of appressorium like structures on plant surfaces. CSG1 is required for posttranscriptional onset of cellulase production after transcriptional induction due to the presence of cellulose or lactose. Acceleration of sexual development in the presence of plant roots and their interactions with fruiting bodies indicates preferential association with plants. Moreover, transcriptome analysis revealed regulation of a sensor essential for cellulase gene expression and secondary metabolite regulation. Our results reveal a complex sensing network governing resource distribution, enzyme production and fungal development that explains previously observed phenomena in fermentations and opens new perspectives for industrial strain improvement and agriculture. Additionally, we found indications that abolishing concentration gated glucose sensing leads to a shift in sensing priorities and metabolic changes highlighting the interconnection between regulation of carbon source utilization, enzyme production and secondary metabolism.

OriginalspracheEnglisch
PublikationsstatusVeröffentlicht - 16 Sep. 2022
Veranstaltung16th Yangling International Agri-science Forum - Northwest A&F University, Department of Science and Technology of Shaaanxi Province, Yangling, China
Dauer: 15 Sep. 202219 Sep. 2022

Seminar/Workshop

Seminar/Workshop16th Yangling International Agri-science Forum
Land/GebietChina
OrtYangling
Zeitraum15/09/2219/09/22

ÖFOS 2012

  • 106022 Mikrobiologie

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