Hexose-6-phosphate dehydrogenase determines the reaction direction of 11beta-hydroxysteroid dehydrogenase type 1 as an oxoreductase

Atanas Atanasov, Lyubomir Nashev, Alex Odermatt, Christoph Frick, Roberto Schweizer

    Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

    Abstract

    The impact of hexose-6-phosphate dehydrogenase (H6PDH) on 11beta-hydroxysteroid dehydrogenase (11beta-HSD) type 1 activity was investigated upon coexpression in HEK-293 cells. Confocal microscopy analysis indicated colocalisation of both enzymes at the lumenal side of the endoplasmic reticulum (ER) membrane. Functional analysis in intact cells revealed fivefold stimulation of 11beta-HSD1 oxoreductase activity and sixfold decrease of dehydrogenase activity upon coexpression with H6PDH, without changing kinetic parameters in cell lysates. Thus, H6PDH directly determines the reaction direction of 11beta-HSD1 in intact cells as an oxoreductase without changing intrinsic catalytic properties of 11beta-HSD1 by regenerating NADPH in the ER-lumen
    OriginalspracheEnglisch
    Seiten (von - bis)129-133
    Seitenumfang5
    FachzeitschriftFEBS Letters
    Jahrgang571
    Ausgabenummer1-3
    PublikationsstatusVeröffentlicht - 2004

    ÖFOS 2012

    • 106023 Molekularbiologie
    • 301114 Zellbiologie

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