HPLC separation of opium alkaloids on porous and non-porous stationary phases

Liselotte Krenn; B Boros; Robert Ohmacht; Laszlo Jelinek

doi:10.1007/BF02492801

HPLC separation of opium alkaloids on porous and non-porous stationary phases

Liselotte Krenn, B Boros, Robert Ohmacht, Laszlo Jelinek

Veröffentlichungen: Beitrag in Fachzeitschrift › Meeting Abstract/Conference Paper

Abstract

A new reversed-phase (RP) HPLC method has been developed and validated for the separation of the main opium alkaloids morphine, codeine, thebaine, papaverine and noscapine on a non-porous (micropellicular) stationary phase. On this phase quantification of the compounds by internal standardization with brucine was achieved extremely rapidly, in ca 1.5 min, only. Thus, the analysis time for the opium alkaloids was approximately one tenth of that on porous stationary phases. Different opium samples were investigated using non-porous and porous packings. The correlation between the results was excellent.

Originalsprache	Englisch
Seiten (von - bis)	S175-S178
Fachzeitschrift	Chromatographia
Jahrgang	51
Ausgabenummer	SUPPL. 2
DOIs	https://doi.org/10.1007/BF02492801
Publikationsstatus	Veröffentlicht - 2000

ÖFOS 2012

3012 Pharmazie, Pharmakologie, Toxikologie

Zugriff auf Dokument

10.1007/BF02492801

Zitationsweisen

@article{20a7feca8f694d838b57056a3d3464fa,

title = "HPLC separation of opium alkaloids on porous and non-porous stationary phases",

abstract = "A new reversed-phase (RP) HPLC method has been developed and validated for the separation of the main opium alkaloids morphine, codeine, thebaine, papaverine and noscapine on a non-porous (micropellicular) stationary phase. On this phase quantification of the compounds by internal standardization with brucine was achieved extremely rapidly, in ca 1.5 min, only. Thus, the analysis time for the opium alkaloids was approximately one tenth of that on porous stationary phases. Different opium samples were investigated using non-porous and porous packings. The correlation between the results was excellent.",

author = "Liselotte Krenn and B Boros and Robert Ohmacht and Laszlo Jelinek",

note = "Coden: CHRGB Affiliations: Institute of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria; Institute of Medical Chemistry, University Medical School of Pecs, Szigeti ut 12, 7624 Pecs, Hungary; Chemie Uetikon AG, 8707 Uetikon, Switzerland Adressen: Krenn, L.; Institute of Pharmacognosy; University of Vienna; Althanstrasse 14 1090 Vienna, Austria Source-File: DirschHeringViernsteinScopus_iso.csv Import aus Scopus: 2-s2.0-0033916243 Importdatum: 29.11.2006 12:36:43 24.08.2007: Datenanforderung 1833 (Import Sachbearbeiter) 22.10.2007: Datenanforderung 1908 (Import Sachbearbeiter)",

year = "2000",

doi = "10.1007/BF02492801",

language = "English",

volume = "51",

pages = "S175--S178",

journal = "Chromatographia",

issn = "0009-5893",

publisher = "Springer-Verlag Berlin-Heidelberg",

number = "SUPPL. 2",

}

TY - JOUR

T1 - HPLC separation of opium alkaloids on porous and non-porous stationary phases

AU - Krenn, Liselotte

AU - Boros, B

AU - Ohmacht, Robert

AU - Jelinek, Laszlo

N1 - Coden: CHRGB Affiliations: Institute of Pharmacognosy, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria; Institute of Medical Chemistry, University Medical School of Pecs, Szigeti ut 12, 7624 Pecs, Hungary; Chemie Uetikon AG, 8707 Uetikon, Switzerland Adressen: Krenn, L.; Institute of Pharmacognosy; University of Vienna; Althanstrasse 14 1090 Vienna, Austria Source-File: DirschHeringViernsteinScopus_iso.csv Import aus Scopus: 2-s2.0-0033916243 Importdatum: 29.11.2006 12:36:43 24.08.2007: Datenanforderung 1833 (Import Sachbearbeiter) 22.10.2007: Datenanforderung 1908 (Import Sachbearbeiter)

PY - 2000

Y1 - 2000

N2 - A new reversed-phase (RP) HPLC method has been developed and validated for the separation of the main opium alkaloids morphine, codeine, thebaine, papaverine and noscapine on a non-porous (micropellicular) stationary phase. On this phase quantification of the compounds by internal standardization with brucine was achieved extremely rapidly, in ca 1.5 min, only. Thus, the analysis time for the opium alkaloids was approximately one tenth of that on porous stationary phases. Different opium samples were investigated using non-porous and porous packings. The correlation between the results was excellent.

AB - A new reversed-phase (RP) HPLC method has been developed and validated for the separation of the main opium alkaloids morphine, codeine, thebaine, papaverine and noscapine on a non-porous (micropellicular) stationary phase. On this phase quantification of the compounds by internal standardization with brucine was achieved extremely rapidly, in ca 1.5 min, only. Thus, the analysis time for the opium alkaloids was approximately one tenth of that on porous stationary phases. Different opium samples were investigated using non-porous and porous packings. The correlation between the results was excellent.

U2 - 10.1007/BF02492801

DO - 10.1007/BF02492801

M3 - Meeting abstract/Conference paper

VL - 51

SP - S175-S178

JO - Chromatographia

JF - Chromatographia

SN - 0009-5893

IS - SUPPL. 2

ER -