Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting

Alessandra Riva, Hamid Rasoulimehrabani, José Manuel Cruz-Rubio, Stephanie L Schnorr, Cornelia von Baeckmann, Deniz Inan, Georgi Nikolov, Craig W Herbold, Bela Hausmann, Petra Pjevac, Arno Schintlmeister, Andreas Spittler, Márton Palatinszky, Aida Kadunic, Norbert Hieger, Giorgia Del Favero, Martin von Bergen, Nico Jehmlich, Margarete Watzka, Kang Soo LeeJulia Wiesenbauer, Sanaz Khadem, Helmut Viernstein, Roman Stocker, Michael Wagner, Christina Kaiser, Andreas Richter, Freddy Kleitz, David Berry (Korresp. Autor*in)

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

Prebiotics are defined as non-digestible dietary components that promote the growth of beneficial gut microorganisms. In many cases, however, this capability is not systematically evaluated. Here, we develop a methodology for determining prebiotic-responsive bacteria using the popular dietary supplement inulin. We first identify microbes with a capacity to bind inulin using mesoporous silica nanoparticles functionalized with inulin. 16S rRNA gene amplicon sequencing of sorted cells revealed that the ability to bind inulin was widespread in the microbiota. We further evaluate which taxa are metabolically stimulated by inulin and find that diverse taxa from the phyla Firmicutes and Actinobacteria respond to inulin, and several isolates of these taxa can degrade inulin. Incubation with another prebiotic, xylooligosaccharides (XOS), in contrast, shows a more robust bifidogenic effect. Interestingly, the Coriobacteriia Eggerthella lenta and Gordonibacter urolithinfaciens are indirectly stimulated by the inulin degradation process, expanding our knowledge of inulin-responsive bacteria.

OriginalspracheEnglisch
Aufsatznummer8210
Seitenumfang15
FachzeitschriftNature Communications
Jahrgang14
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - Dez. 2023

ÖFOS 2012

  • 106026 Ökosystemforschung
  • 106022 Mikrobiologie

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