Inhibitory effects of C2 to C10 1-alkynes on ammonia oxidation in two Nitrososphaera species

A. E. Taylor, K. Taylor, B. Tennigkeit, Marton Palatinszky, Michaela Stieglmeier, D. D. Myrold, Christa Schleper, Michael Wagner, P. J. Bottomley

    Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

    Abstract

    A previous study showed that ammonia oxidation by the Thaumarchaeota Nitrosopumilus maritimus (group 1.1a) was resistant to concentrations of the C 8 1-alkyne, octyne, which completely inhibits activity by ammonia-oxidizing bacteria. In this study, the inhibitory effects of octyne and other C 2 to C 10 1-alkynes were evaluated on the nitrite production activity of two pure culture isolates from Thaumarchaeota group 1.1b, Nitrososphaera viennensis strain EN76 and Nitrososphaera gargensis. Both N. viennensis and N. gargensis were insensitive to concentrations of octyne that cause complete and irreversible inactivation of nitrite production by ammonia-oxidizing bacteria. However, octyne concentrations (≥20 μM) that did not inhibit N. maritimus partially inhibited nitrite production in N. viennensis and N. gargensis in a manner that did not show the characteristics of irreversible inactivation. In contrast to previous studies with an ammonia-oxidizing bacterium, Nitrosomonas europaea, octyne inhibition of N. viennensis was: (i) fully and immediately reversible, (ii) not competitive with NH 4 +, and (iii) without effect on the competitive interaction between NH 4 + and acetylene. Both N. viennensis and N. gargensis demonstrated the same overall trend in regard to 1-alkyne inhibition as previously observed for N. maritimus, being highly sensitive to ≤C 5 alkynes and more resistant to longer-chain length alkynes. Reproducible differences were observed among N. maritimus, N. viennensis, and N. gargensis in regard to the extent of their resistance/sensitivity to C 6 and C 7 1-alkynes, which may indicate differences in the ammonia monooxygenase binding and catalytic site(s) among the Thaumarchaeota.

    OriginalspracheEnglisch
    Seiten (von - bis)1942-1948
    Seitenumfang7
    FachzeitschriftApplied and Environmental Microbiology
    Jahrgang81
    Ausgabenummer6
    DOIs
    PublikationsstatusVeröffentlicht - März 2015

    ÖFOS 2012

    • 106022 Mikrobiologie

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