TY - JOUR
T1 - Linear element-independent meiotic recombination in Schizosaccharomyces pombe
AU - Wells, Jennifer L.
AU - Pryce, David W.
AU - Estreicher, Anna
AU - Loidl, Josef
AU - McFarlane, Ramsay J.
N1 - DOI: 10.1534/genetics.106.063818
Coden: GENTA
Affiliations: North West Cancer Research Fund Institute, University of Wales, Bangor, LL57 2UW, United Kingdom; Department of Chromosome Biology, University of Vienna, A-1030, Vienna, Austria; North West Cancer Research Fund Institute, University of Wales, Memorial Bldg., Deiniol Rd., Bangor, Gwynedd, LL57 2UW, United Kingdom
Adressen: McFarlane, R.J.; North West Cancer Research Fund Institute; University of Wales; Memorial Bldg.; Deiniol Rd. Bangor, Gwynedd, LL57 2UW, United Kingdom; email: [email protected]
Source-File: Bio562Scopus.csv
Import aus Scopus: 2-s2.0-33751352090
Importdatum: 25.01.2007 12:59:11
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
PY - 2006
Y1 - 2006
N2 - Most organisms form protein-rich, linear, ladder-like structures associated with chromosomes during early meiosis, the synaptonemal complex. In Schizosaccharomyces pombe, linear elements (LinEs) are thread-like, proteinacious chromosome-associated structures that form during early meiosis. LinEs are related to axial elements, the synaptonemal complex precursors of other organisms. Previous studies have led to the suggestion that axial structures are essential to mediate meiotic recombination. Rec10 protein is a major component of S. pombe LinEs and is required for their development. In this report we study recombination in a number of rec10 mutants, one of which (rec10-155) does not form LinEs, but is predicted to encode a truncated Rec10 protein. This mutant has levels of crossing over and gene conversion substantially higher than a rec10 null mutant (rec10-175) and forms cytologically detectable Rad51 foci indicative of meiotic recombination intermediates. These data demonstrate that while Rec10 is required for meiotic recombination, substantial meiotic recombination can occur in rec10 mutants that do not form LinEs, indicating that LinEs per se are not essential for all meiotic recombination. Copyright Œ 2006 by the Genetics Society of America.
AB - Most organisms form protein-rich, linear, ladder-like structures associated with chromosomes during early meiosis, the synaptonemal complex. In Schizosaccharomyces pombe, linear elements (LinEs) are thread-like, proteinacious chromosome-associated structures that form during early meiosis. LinEs are related to axial elements, the synaptonemal complex precursors of other organisms. Previous studies have led to the suggestion that axial structures are essential to mediate meiotic recombination. Rec10 protein is a major component of S. pombe LinEs and is required for their development. In this report we study recombination in a number of rec10 mutants, one of which (rec10-155) does not form LinEs, but is predicted to encode a truncated Rec10 protein. This mutant has levels of crossing over and gene conversion substantially higher than a rec10 null mutant (rec10-175) and forms cytologically detectable Rad51 foci indicative of meiotic recombination intermediates. These data demonstrate that while Rec10 is required for meiotic recombination, substantial meiotic recombination can occur in rec10 mutants that do not form LinEs, indicating that LinEs per se are not essential for all meiotic recombination. Copyright Œ 2006 by the Genetics Society of America.
M3 - Article
SN - 0016-6731
VL - 174
SP - 1105
EP - 1114
JO - Genetics: a periodical record of investigations bearing on heredity and variation
JF - Genetics: a periodical record of investigations bearing on heredity and variation
IS - 3
ER -