TY - JOUR
T1 - Lotus burttii takes a position of the third corner in the Lotus molecular genetics triangle
AU - Kawaguchi, Masayoshi
AU - Pedrosa Harand, Andrea
AU - Yano, Koji
AU - Hayashi, Makoto
AU - Murooka, Yoshikatsu
AU - Saito, Katsuharu
AU - Nagata, Toshiyuki
AU - Namai, Kiyoshi
AU - Nishida, Hiroshi
AU - Shibata, Daisuke
AU - Sato, Shusei
AU - Tabata, Satoshi
AU - Hayashi, Masaki
AU - Harada, Kyuya
AU - Sandal, Niels
AU - Stougaard, Jens
AU - Bachmair, Andreas
AU - Grant, William F.
N1 - Zeitschrift: DNA Research
Coden: DARSE
Affiliations: Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan; Dept. of Cell Biology and Genetics, Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria; Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamdaoka, Suita, Osaka 565-0871, Japan; Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan; Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark; Department of Plant Science, Macdonald Camp. of McGill University, Ste. Anne de Bellevue, Que. H9X 3V9, Canada; Faculty of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba, 271-8510, Japan; Core Res. Evolutional Sci. Technol., Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0112, Japan; Tochigi Pref. Agric. Experiment, St., 1,080 Kawaraya-cho, Utsunomiya 320-0002, Japan; Dept. of Plant Developmental Biology, Max Planck Inst. Plant Breed. Res., Carl-von-LinneŽ-Weg 10, D-50829 Cologne, Germany
Adressen: Kawaguchi, M.; Department of Biological Sciences; Graduate School of Science; University of Tokyo; 7-3-1 Hongo Bunkyo, Tokyo 113-0033, Japan; email: [email protected]
Source-File: Bio562Scopus.csv
Import aus Scopus: 2-s2.0-20944446058
Importdatum: 25.01.2007 12:59:22
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
PY - 2005
Y1 - 2005
N2 - In order to consolidate molecular genetic system in Lotus japonicus and to further access the biological diversity in Lotea, we introduce here Lotus burttii B-303 derived from West Pakistan as the third crossing partner of the Gifu ecotype (B-129-S9) for a genetic analysis. L. burttii is a relatively small and early flowering plant with non-shattering behavior. The general chromosome morphology is very similar to Gifu, and fluorescence in situ hybridization (FISH) analysis revealed that the short arm of chromosome 1 in L. burttii is comparable to that of Gifu, indicating that the translocation event involving chromosomes 1 and 2, which was observed in L. japonicus Miyakojima MG-20, is not present in L. burttii. In addition L. burttii has a higher level of DNA polymorphism compared to Gifu and MG-20 enabling design of codominant markers such as SSR, CAPS and dCAPS. Using an F2 population from a cross between Gifu and L. burttii, codominant makers that co-segregated at the translocation site could be expanded. In order to normalize the genetic background, L. burttii was inbred for nine generations and the germplasm L. burttii B-303-S9 was established.
AB - In order to consolidate molecular genetic system in Lotus japonicus and to further access the biological diversity in Lotea, we introduce here Lotus burttii B-303 derived from West Pakistan as the third crossing partner of the Gifu ecotype (B-129-S9) for a genetic analysis. L. burttii is a relatively small and early flowering plant with non-shattering behavior. The general chromosome morphology is very similar to Gifu, and fluorescence in situ hybridization (FISH) analysis revealed that the short arm of chromosome 1 in L. burttii is comparable to that of Gifu, indicating that the translocation event involving chromosomes 1 and 2, which was observed in L. japonicus Miyakojima MG-20, is not present in L. burttii. In addition L. burttii has a higher level of DNA polymorphism compared to Gifu and MG-20 enabling design of codominant markers such as SSR, CAPS and dCAPS. Using an F2 population from a cross between Gifu and L. burttii, codominant makers that co-segregated at the translocation site could be expanded. In order to normalize the genetic background, L. burttii was inbred for nine generations and the germplasm L. burttii B-303-S9 was established.
M3 - Article
SN - 1340-2838
VL - 12
SP - 69
EP - 77
JO - DNA Research
JF - DNA Research
IS - 1
ER -