TY - JOUR
T1 - Meiotic telomere clustering requires actin for its formation and cohesin for its resolution
AU - Trelles-Sticken, Edgar
AU - Adelfalk, Caroline
AU - Loidl, Josef
AU - Scherthan, Harry
N1 - DOI: 10.1083/jcb.200501042
Coden: JCLBA
Affiliations: Max-Planck-Institute for Molecular Genetics, D-14195 Berlin, Germany; Department of Chromosome Biology, Faculty of Life Sciences, University of Vienna, A-1030 Vienna, Austria; Institute for Radiation Biology Bundeswehr, D-80937 Munich, Germany; Schuetzenstrasse 31, D-12105 Berlin, Germany
Adressen: Scherthan, H.; Max-Planck-Institute for Molecular Genetics D-14195 Berlin, Germany; email: [email protected]
Source-File: Bio562Scopus.csv
Import aus Scopus: 2-s2.0-22944433512
Importdatum: 25.01.2007 12:59:03
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
PY - 2005
Y1 - 2005
N2 - In diploid organisms, meiosis reduces the chromosome number by half during the formation of haploid gametes. During meiotic prophase, telomeres transiently cluster at a limited sector of the nuclear envelope (bouquet stage) near the spindle pole body (SPB). Cohesin is a multisubunit complex that contributes to chromosome segregation in meiosis I and II divisions. In yeast meiosis, deficiency for Rec8 cohesin subunit induces telomere clustering to persist, whereas telomere cluster-SPB colocalization is defective. These defects are rescued by expressing the mitotic cohesin Scc1 in rec8? meiosis, whereas bouquet-stage exit is independent of Cdc5 pololike kinase. An analysis of living Saccharomyces cerevisiae meiocytes revealed highly mobile telomeres from leptotene up to pachytene, with telomeres experiencing an actin- but not microtubule-dependent constraint of mobility during the bouquet stage. Our results suggest that cohesin is required for exit from actin polymerization-dependent telomere clustering and for linking the SPB to the telomere cluster in synaptic meiosis. Œ The Rockefeller University Press.
AB - In diploid organisms, meiosis reduces the chromosome number by half during the formation of haploid gametes. During meiotic prophase, telomeres transiently cluster at a limited sector of the nuclear envelope (bouquet stage) near the spindle pole body (SPB). Cohesin is a multisubunit complex that contributes to chromosome segregation in meiosis I and II divisions. In yeast meiosis, deficiency for Rec8 cohesin subunit induces telomere clustering to persist, whereas telomere cluster-SPB colocalization is defective. These defects are rescued by expressing the mitotic cohesin Scc1 in rec8? meiosis, whereas bouquet-stage exit is independent of Cdc5 pololike kinase. An analysis of living Saccharomyces cerevisiae meiocytes revealed highly mobile telomeres from leptotene up to pachytene, with telomeres experiencing an actin- but not microtubule-dependent constraint of mobility during the bouquet stage. Our results suggest that cohesin is required for exit from actin polymerization-dependent telomere clustering and for linking the SPB to the telomere cluster in synaptic meiosis. Œ The Rockefeller University Press.
M3 - Article
SN - 0021-9525
VL - 170
SP - 213
EP - 223
JO - The Journal of Cell Biology (JCB)
JF - The Journal of Cell Biology (JCB)
IS - 2
ER -