TY - JOUR
T1 - Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase
AU - Penkner, Alexandra
AU - Prinz, Susanne
AU - Ferscha, Stefan
AU - Klein, Franz
N1 - DOI: 10.1016/j.cell.2005.01.017
Coden: CELLB
Affiliations: Vienna Biocenter II, Max Perutz Laboratories, Department of Chromosome Biology, Dr. Bohr-Gasse 1, A-1030 Vienna, Austria; Institute for Systems Biology, 1441 N. 34thStreet, Seattle, WA 98103, United States
Adressen: Klein, F.; Vienna Biocenter II; Max Perutz Laboratories; Department of Chromosome Biology; Dr. Bohr-Gasse 1 A-1030 Vienna, Austria; email: [email protected]
Source-File: MFPLUniWienScopus.csv
Import aus Scopus: 2-s2.0-17644373803
Importdatum: 07.12.2006 15:13:16
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
PY - 2005
Y1 - 2005
N2 - Meiotic cohesin serves in sister chromatid linkage and DNA repair until its subunit Rec8 is cleaved by separase. Separase is activated when its inhibitor, securin, is polyubiquitinated by the Cdc20 regulated anaphase-promoting complex (APCCdc20) and consequently degraded. Differently regulated APCs (APCCdh1, APCAma1) have not been implicated in securin degradation at meiosis I. We show that Mnd2, a factor known to associate with APC components, prevents premature securin degradation in meiosis by APC Ama1. mnd2? cells lack linear chromosome axes and exhibit precocious sister chromatid separation, but deletion of AMA1 suppresses these defects. Besides securin, Sgo1, a protein essential for protection of centromeric cohesion during anaphase I, is also destabilized in mnd2? cells. Mnd2's disappearance prior to anaphase II may activate APC Ama1. Human oocytes may spend many years in meiotic prophase before maturation. Inhibitors of meiotic APC variants could prevent loss of chiasmata also in these cells, thereby guarding against aberrant chromosome segregation. Copyright Œ2005 by Elsevier Inc.
AB - Meiotic cohesin serves in sister chromatid linkage and DNA repair until its subunit Rec8 is cleaved by separase. Separase is activated when its inhibitor, securin, is polyubiquitinated by the Cdc20 regulated anaphase-promoting complex (APCCdc20) and consequently degraded. Differently regulated APCs (APCCdh1, APCAma1) have not been implicated in securin degradation at meiosis I. We show that Mnd2, a factor known to associate with APC components, prevents premature securin degradation in meiosis by APC Ama1. mnd2? cells lack linear chromosome axes and exhibit precocious sister chromatid separation, but deletion of AMA1 suppresses these defects. Besides securin, Sgo1, a protein essential for protection of centromeric cohesion during anaphase I, is also destabilized in mnd2? cells. Mnd2's disappearance prior to anaphase II may activate APC Ama1. Human oocytes may spend many years in meiotic prophase before maturation. Inhibitors of meiotic APC variants could prevent loss of chiasmata also in these cells, thereby guarding against aberrant chromosome segregation. Copyright Œ2005 by Elsevier Inc.
U2 - 10.1016/j.cell.2005.01.017
DO - 10.1016/j.cell.2005.01.017
M3 - Article
SN - 0092-8674
VL - 120
SP - 789
EP - 801
JO - Cell
JF - Cell
IS - 6
ER -