Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

Werner Praznik; Andrea Cavarkapa; Frank M. Unger; Renate Loeppert; Wolfgang Holzer; Helmut Viernstein; Monika Mueller

doi:10.1016/j.foodchem.2016.11.087

Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

Werner Praznik (Korresp. Autor*in), Andrea Cavarkapa, Frank M. Unger, Renate Loeppert, Wolfgang Holzer, Helmut Viernstein, Monika Mueller

Veröffentlichungen: Beitrag in Fachzeitschrift › Artikel › Peer Reviewed

Abstract

The seed mucilage of Hyptis suaveolens L. includes acid – and neutral heteropolysaccharides in a ratio of
about 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an average
molar mass of Mw = 350 kg/mol, Mn = 255 kg/mol. The neutral polysaccharide fraction shows an
average molar mass of Mw = 47 kg/mol and Mn = 28 kg/mol and is composed of D-Galp-, D-Glcp- and
D-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) and
galactoglucomannan (70%) with a high level of terminal b-linked D-Galp residues (18%). Structural details
of galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMR
spectroscopy. Sequences of octa/nonasaccharide b-D-Glcp-(1 ?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-
b-D-Manp-(1?4)-b-D-Glcp-(1?4)-b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp and
lower mass tetrasaccharide repeating units b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-DManp
were found. The level of the prebiotic activity is related to the availability of b-linked D-Galp residues
in the side chains of the molecules.

Originalsprache	Englisch
Seiten (von - bis)	1997-2004
Seitenumfang	8
Fachzeitschrift	Food Chemistry
Jahrgang	221
DOIs	https://doi.org/10.1016/j.foodchem.2016.11.087
Publikationsstatus	Veröffentlicht - 15 Apr. 2017

ÖFOS 2012

301208 Pharmazeutische Technologie
104013 Naturstoffchemie

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10.1016/j.foodchem.2016.11.087

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http://www.scopus.com/inward/record.url?scp=85006778773&partnerID=8YFLogxK

Zitationsweisen

@article{551f1464754a42e385b29e945505b8f3,

title = "Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.",

abstract = "The seed mucilage of Hyptis suaveolens L. includes acid – and neutral heteropolysaccharides in a ratio ofabout 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an averagemolar mass of Mw = 350 kg/mol, Mn = 255 kg/mol. The neutral polysaccharide fraction shows anaverage molar mass of Mw = 47 kg/mol and Mn = 28 kg/mol and is composed of D-Galp-, D-Glcp- andD-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) andgalactoglucomannan (70%) with a high level of terminal b-linked D-Galp residues (18%). Structural detailsof galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMRspectroscopy. Sequences of octa/nonasaccharide b-D-Glcp-(1 ?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp-(1?4)-b-D-Glcp-(1?4)-b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp andlower mass tetrasaccharide repeating units b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-DManpwere found. The level of the prebiotic activity is related to the availability of b-linked D-Galp residuesin the side chains of the molecules.",

keywords = "Galactoglucan, Galactoglucomannan, Hyptis suaveolens L., Methylation analysis, Mucilage, NMR spectroscopy, Polysaccharide, GALACTOGLUCOMANNAN",

author = "Werner Praznik and Andrea Cavarkapa and Unger, {Frank M.} and Renate Loeppert and Wolfgang Holzer and Helmut Viernstein and Monika Mueller",

year = "2017",

month = apr,

day = "15",

doi = "10.1016/j.foodchem.2016.11.087",

language = "English",

volume = "221",

pages = "1997--2004",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "ELSEVIER SCI LTD",

}

TY - JOUR

T1 - Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

AU - Praznik, Werner

AU - Cavarkapa, Andrea

AU - Unger, Frank M.

AU - Loeppert, Renate

AU - Holzer, Wolfgang

AU - Viernstein, Helmut

AU - Mueller, Monika

PY - 2017/4/15

Y1 - 2017/4/15

N2 - The seed mucilage of Hyptis suaveolens L. includes acid – and neutral heteropolysaccharides in a ratio ofabout 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an averagemolar mass of Mw = 350 kg/mol, Mn = 255 kg/mol. The neutral polysaccharide fraction shows anaverage molar mass of Mw = 47 kg/mol and Mn = 28 kg/mol and is composed of D-Galp-, D-Glcp- andD-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) andgalactoglucomannan (70%) with a high level of terminal b-linked D-Galp residues (18%). Structural detailsof galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMRspectroscopy. Sequences of octa/nonasaccharide b-D-Glcp-(1 ?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp-(1?4)-b-D-Glcp-(1?4)-b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp andlower mass tetrasaccharide repeating units b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-DManpwere found. The level of the prebiotic activity is related to the availability of b-linked D-Galp residuesin the side chains of the molecules.

AB - The seed mucilage of Hyptis suaveolens L. includes acid – and neutral heteropolysaccharides in a ratio ofabout 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an averagemolar mass of Mw = 350 kg/mol, Mn = 255 kg/mol. The neutral polysaccharide fraction shows anaverage molar mass of Mw = 47 kg/mol and Mn = 28 kg/mol and is composed of D-Galp-, D-Glcp- andD-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) andgalactoglucomannan (70%) with a high level of terminal b-linked D-Galp residues (18%). Structural detailsof galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMRspectroscopy. Sequences of octa/nonasaccharide b-D-Glcp-(1 ?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp-(1?4)-b-D-Glcp-(1?4)-b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-D-Manp andlower mass tetrasaccharide repeating units b-D-Glcp-(1?4)[b-D-Galp-(1?2)-a-D-Galp-(1?6)]-b-DManpwere found. The level of the prebiotic activity is related to the availability of b-linked D-Galp residuesin the side chains of the molecules.

KW - Galactoglucan

KW - Galactoglucomannan

KW - Hyptis suaveolens L.

KW - Methylation analysis

KW - Mucilage

KW - NMR spectroscopy

KW - Polysaccharide

KW - GALACTOGLUCOMANNAN

UR - http://www.scopus.com/inward/record.url?scp=85006778773&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2016.11.087

DO - 10.1016/j.foodchem.2016.11.087

M3 - Article

AN - SCOPUS:85006778773

SN - 0308-8146

VL - 221

SP - 1997

EP - 2004

JO - Food Chemistry

JF - Food Chemistry

ER -

Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.

Abstract

ÖFOS 2012

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