Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying S. epidermidis

Chularat Hlaoperm, Wisnu Arfian A. Sudjarwo, Jakob Ehrenbrandtner, Endre Kiss, Giorgia Del Favero, Kiattawee Choowongkomon, Jatuporn Rattanasrisomporn (Korresp. Autor*in), Peter A. Lieberzeit (Korresp. Autor*in)

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

Staphylococcus epidermidis (S. epidermidis) belongs to methicillin-resistant bacteria strains that cause severe disease in humans. Herein, molecularly imprinted polymer (MIP) nanoparticles resulting from solid-phase synthesis on entire cells were employed as a sensing material to identify the species. MIP nanoparticles revealed spherical shapes with diameters of approximately 70 nm to 200 nm in scanning electron microscopy (SEM), which atomic force microscopy (AFM) confirmed. The interaction between nanoparticles and bacteria was assessed using height image analysis in AFM. Selective binding between MIP nanoparticles and S. epidermidis leads to uneven surfaces on bacteria. The surface roughness of S. epidermidis cells was increased to approximately 6.3 ± 1.2 nm after binding to MIP nanoparticles from around 1 nm in the case of native cells. This binding behavior is selective: when exposing Escherichia coli and Bacillus subtilis to the same MIP nanoparticle solutions, one cannot observe binding. Fluorescence microscopy confirms both sensitivity and selectivity. Hence, the developed MIP nanoparticles are a promising approach to identify (pathogenic) bacteria species.

OriginalspracheEnglisch
Aufsatznummer3526
FachzeitschriftSensors
Jahrgang23
Ausgabenummer7
DOIs
PublikationsstatusVeröffentlicht - Apr. 2023

ÖFOS 2012

  • 104017 Physikalische Chemie
  • 202036 Sensorik

Fingerprint

Untersuchen Sie die Forschungsthemen von „Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying S. epidermidis“. Zusammen bilden sie einen einzigartigen Fingerprint.

Zitationsweisen