Monopolar Attachment of Sister Kinetochores at Meiosis I Requires Casein Kinase 1

Mark Petronczki, Joao Matos, Saori Mori, Juraj Gregan, Aliona I. Bogdanova, Martin Schwickart, Karl Mechtler, Katsuhiko Shirahige, Wolfgang Zachariae, Kim Nasmyth

    Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

    Abstract

    In meiosis, a single round of DNA replication is followed by two consecutive rounds of chromosome segregation, called meiosis I and II. Disjunction of maternal from paternal centromeres during meiosis I depends on the attachment of sister kinetochores to microtubules emanating from the same pole. In budding yeast, monopolar attachment requires recruitment to kinetochores of the monopolin complex. How monopolin promotes monopolar attachment was unclear, as its subunits are poorly conserved and lack similarities to proteins with known functions. We show here that the monopolin subunit Mam1 binds tightly to Hrr25, a highly conserved casein kinase 1 d/e (CK1d/e), and recruits it to meiosis I centromeres. Hrr25 kinase activity and Mam1 binding are both essential for monopolar attachment. Since CK1d/e activity is important for accurate chromosome segregation during meiosis I also in fission yeast, phosphorylation of kinetochore proteins by CK1d/e might be an evolutionary conserved process required for monopolar attachment. Œ 2006 Elsevier Inc. All rights reserved.
    OriginalspracheEnglisch
    Seiten (von - bis)1049-1064
    Seitenumfang16
    FachzeitschriftCell
    Jahrgang126
    Ausgabenummer6
    PublikationsstatusVeröffentlicht - 2006

    ÖFOS 2012

    • 1060 Biologie

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