TY - JOUR
T1 - Monopolar Attachment of Sister Kinetochores at Meiosis I Requires Casein Kinase 1
AU - Petronczki, Mark
AU - Matos, Joao
AU - Mori, Saori
AU - Gregan, Juraj
AU - Bogdanova, Aliona I.
AU - Schwickart, Martin
AU - Mechtler, Karl
AU - Shirahige, Katsuhiko
AU - Zachariae, Wolfgang
AU - Nasmyth, Kim
N1 - DOI: 10.1016/j.cell.2006.07.029
Coden: CELLB
Affiliations: Research Institute of Molecular Pathology, Dr. Bohrgasse 7, A-1030 Vienna, Austria; Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany; Laboratory of Genome Structure and Function, Division of Gene Research, Center for Biological Resources and Informatics, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan; Genentech Inc., 1 DNA Way, South San Francisco, CA 94080, United States; Department of Biochemistry, University of Oxford, South Parks Road, OX1 3QU Oxford, United Kingdom
Adressen: Zachariae, W.; Max Planck Institute of Molecular Cell Biology and Genetics; Pfotenhauerstrasse 108 01307 Dresden, Germany; email: [email protected]
Source-File: MFPLUniWienScopus.csv
Import aus Scopus: 2-s2.0-33748607719
Importdatum: 07.12.2006 15:09:45
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
PY - 2006
Y1 - 2006
N2 - In meiosis, a single round of DNA replication is followed by two consecutive rounds of chromosome segregation, called meiosis I and II. Disjunction of maternal from paternal centromeres during meiosis I depends on the attachment of sister kinetochores to microtubules emanating from the same pole. In budding yeast, monopolar attachment requires recruitment to kinetochores of the monopolin complex. How monopolin promotes monopolar attachment was unclear, as its subunits are poorly conserved and lack similarities to proteins with known functions. We show here that the monopolin subunit Mam1 binds tightly to Hrr25, a highly conserved casein kinase 1 d/e (CK1d/e), and recruits it to meiosis I centromeres. Hrr25 kinase activity and Mam1 binding are both essential for monopolar attachment. Since CK1d/e activity is important for accurate chromosome segregation during meiosis I also in fission yeast, phosphorylation of kinetochore proteins by CK1d/e might be an evolutionary conserved process required for monopolar attachment. Œ 2006 Elsevier Inc. All rights reserved.
AB - In meiosis, a single round of DNA replication is followed by two consecutive rounds of chromosome segregation, called meiosis I and II. Disjunction of maternal from paternal centromeres during meiosis I depends on the attachment of sister kinetochores to microtubules emanating from the same pole. In budding yeast, monopolar attachment requires recruitment to kinetochores of the monopolin complex. How monopolin promotes monopolar attachment was unclear, as its subunits are poorly conserved and lack similarities to proteins with known functions. We show here that the monopolin subunit Mam1 binds tightly to Hrr25, a highly conserved casein kinase 1 d/e (CK1d/e), and recruits it to meiosis I centromeres. Hrr25 kinase activity and Mam1 binding are both essential for monopolar attachment. Since CK1d/e activity is important for accurate chromosome segregation during meiosis I also in fission yeast, phosphorylation of kinetochore proteins by CK1d/e might be an evolutionary conserved process required for monopolar attachment. Œ 2006 Elsevier Inc. All rights reserved.
M3 - Article
SN - 0092-8674
VL - 126
SP - 1049
EP - 1064
JO - Cell
JF - Cell
IS - 6
ER -