Novel genes required for meiotic chromosome segregation are identified by a high-throughput knockout screen in fission yeast

Juraj Gregan, Peter K. Rabitsch, Ortansa Csutak, Vitaly Latypov, Elisabeth Lehmann, Jürg Kohli, Kim Nasmyth

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

Two rounds of chromosome segregation after only a single round of DNA replication enable the production of haploid gametes from diploid precursors during meiosis. To identify genes involved in meiotic chromosome segregation, we developed an efficient strategy to knock out genes in the fission yeast on a large scale. We used this technique to delete 180 functionally uncharacterized genes whose expression is upregulated during meiosis. Deletion of two genes, sgo1 and mde2, caused massive chromosome missegregation. sgo1 is required for retention of centromeric sister-chromatid cohesion after anaphase I [1-3]. We show here that mde2 is required for formation of the double-strand breaks necessary for meiotic recombination. Œ2005 Elsevier Ltd. All rights reserved.
OriginalspracheEnglisch
Seiten (von - bis)1663-1669
Seitenumfang7
FachzeitschriftCurrent Biology
Jahrgang15
Ausgabenummer18
PublikationsstatusVeröffentlicht - 2005

ÖFOS 2012

  • 1060 Biologie

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