On the fate of skeletal myoblasts in a cardiac environment: Down-regulation of voltage-gated ion channels

H C Ott, Stanislav Berjukow, Rainer Marksteiner, Eva Margreiter, G Böck, Günter Laufer, Steffen Hering

    Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

    Abstract

    We have analysed the voltage-gated ion channels and fusion competence of skeletal muscle myoblasts labelled with green fluorescent protein (GFP) and the membrane dye PKH transplanted into the infarcted myocardium of syngenic rats. After cell transplantation the animals were killed and GFP+ - PKH+ myoblasts enzymatically isolated for subsequent studies of ionic currents through voltage-gated sodium, calcium and potassium channels. A down-regulation of all three types of ion channels after engraftment was observed. The fraction of cells with calcium (68%) and sodium channels (65%) declined to zero within 24 h and 1 week, respectively. Down-regulation of potassium currents (90% in control) occurred within 2 weeks to about 30%. Before injection myoblasts expressed predominantly transient outward potassium channels whereas after isolation from the myocardium exclusively rapid delayed rectifier channels. The currents recovered completely between 1 and 6 weeks under cell culture conditions. The down-regulation of ion channels and changes in potassium current kinetics suggest that the environment provided by infarcted myocardium affects expression of voltage-gated ion channels of skeletal myoblasts. ΠThe Physiological Society 2004.
    OriginalspracheEnglisch
    Seiten (von - bis)793-805
    Seitenumfang13
    FachzeitschriftJournal of Physiology (Paris)
    Jahrgang558
    Ausgabenummer3
    PublikationsstatusVeröffentlicht - 2004

    ÖFOS 2012

    • 3012 Pharmazie, Pharmakologie, Toxikologie

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