Physiology and methane productivity of Methanobacterium thermaggregans

Lisa-Maria Mauerhofer, Barbara Reischl, Tilman Schmider, Benjamin Schupp, Kinga Nagy, Patricia Pappenreiter, Sara Zwirtmayr, Bernhard Schuster, Sébastien Bernacchi, Arne H Seifert, Christian Paulik, Simon K-M R Rittmann

    Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

    Abstract

    Accumulation of carbon dioxide (CO2), associated with global temperature rise, and drastically decreasing fossil fuels necessitate the development of improved renewable and sustainable energy production processes. A possible route for CO2 recycling is to employ autotrophic and hydrogenotrophic methanogens for CO2-based biological methane (CH4) production (CO2-BMP). In this study, the physiology and productivity of Methanobacterium thermaggregans was investigated in fed-batch cultivation mode. It is shown that M. thermaggregans can be reproducibly adapted to high agitation speeds for an improved CH4 productivity. Moreover, inoculum size, sulfide feeding, pH, and temperature were optimized. Optimization of growth and CH4 productivity revealed that M. thermaggregans is a slightly alkaliphilic and thermophilic methanogen. Hitherto, it was only possible to grow seven autotrophic, hydrogenotrophic methanogenic strains in fed-batch cultivation mode. Here, we show that after a series of optimization and growth improvement attempts another methanogen, M. thermaggregas could be adapted to be grown in fed-batch cultivation mode to cell densities of up to 1.56 g L-1. Moreover, the CH4 evolution rate (MER) of M. thermaggregans was compared to Methanothermobacter marburgensis, the CO2-BMP model organism. Under optimized cultivation conditions, a maximum MER of 96.1 ± 10.9 mmol L-1 h-1 was obtained with M. thermaggregans-97% of the maximum MER that was obtained utilizing M. marburgensis in a reference experiment. Therefore, M. thermaggregans can be regarded as a CH4 cell factory highly suited to be applicable for CO2-BMP.

    OriginalspracheEnglisch
    Seiten (von - bis)7643-7656
    Seitenumfang14
    FachzeitschriftApplied Microbiology and Biotechnology
    Jahrgang102
    Ausgabenummer17
    DOIs
    PublikationsstatusVeröffentlicht - Sep. 2018

    ÖFOS 2012

    • 209003 Biotreibstoffe

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