PPARγ Modulates Long Chain Fatty Acid Processing in the Intestinal Epithelium

Kalina Duszka, Matej Oresic, Cedric Le May, Jürgen König, Walter Wahli

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

Nuclear receptor PPARγ affects lipid metabolism in several tissues, but its role in intestinal lipid metabolism has not been explored. As alterations have been observed in the plasma lipid profile of ad libitum fed intestinal epithelium-specific PPARγ knockout mice (iePPARγKO), we submitted these mice to lipid gavage challenges. Within hours after gavage with long chain unsaturated fatty acid (FA)-rich canola oil, the iePPARγKO mice had higher plasma free FA levels and lower gastric inhibitory polypeptide levels than their wild-type (WT) littermates, and altered expression of incretin genes and lipid metabolism-associated genes in the intestinal epithelium. Gavage with the medium chain saturated FA-rich coconut oil did not result in differences between the two genotypes. Furthermore, the iePPARγKO mice did not exhibit defective lipid uptake and stomach emptying; however, their intestinal transit was more rapid than in WT mice. When fed a canola oil-rich diet for 4.5 months, iePPARγKO mice had higher body lean mass than the WT mice. We conclude that intestinal epithelium PPARγ is activated preferentially by long chain unsaturated FAs compared to medium chain saturated FAs. Furthermore, we hypothesize that the iePPARγKO phenotype originates from altered lipid metabolism and release in epithelial cells, as well as changes in intestinal motility.

OriginalspracheEnglisch
Aufsatznummer2559
Seitenumfang15
FachzeitschriftInternational Journal of Molecular Sciences
Jahrgang18
Ausgabenummer12
DOIs
PublikationsstatusVeröffentlicht - Dez. 2017

ÖFOS 2012

  • 303009 Ernährungswissenschaften

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