Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I

Christian G. Riedel, Vittorio L. Katis, Yuki Mori Katou, Saori Mori, Takehiko Itoh, Wolfgang Helmhart, Marta Galova, Mark Petronczki, Juraj Gregan, Bulent Cetin, Ingrid Mudrak, Egon Ogris, Karl Mechtler, Laurence Pelletier, Frank Buchholz, Katsuhiko Shirahige, Kim Nasmyth

    Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

    Abstract

    Segregation of homologous maternal and paternal centromeres to opposite poles during meiosis I depends on post-replicative crossing over between homologous non-sister chromatids, which creates chiasmata and therefore bivalent chromosomes. Destruction of sister chromatid cohesion along chromosome arms due to proteolytic cleavage of cohesin's Rec8 subunit by separase resolves chiasmata and thereby triggers the first meiotic division. This produces univalent chromosomes, the chromatids of which are held together by centromeric cohesin that has been protected from separase by shugoshin (Sgo1/MEI-S332) proteins. Here we show in both fission and budding yeast that Sgo1 recruits to centromeres a specific form of protein phosphatase 2A (PP2A). Its inactivation causes loss of centromeric cohesin at anaphase I and random segregation of sister centromeres at the second meiotic division. Artificial recruitment of PP2A to chromosome arms prevents Rec8 phosphorylation and hinders resolution of chiasmata. Our data are consistent with the notion that efficient cleavage of Rec8 requires phosphorylation of cohesin and that this is blocked by PP2A at meiosis I centromeres. Œ 2006 Nature Publishing Group.
    OriginalspracheEnglisch
    Seiten (von - bis)53-61
    Seitenumfang9
    FachzeitschriftNature
    Jahrgang441
    Ausgabenummer1
    DOIs
    PublikationsstatusVeröffentlicht - 2006

    ÖFOS 2012

    • 1060 Biologie

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