TY - JOUR
T1 - RNA aptamers binding the double-stranded RNA-binding domain
AU - Hallegger, Eva-Martina
AU - Taschner, Andreas
AU - Jantsch, Franz-Michael
N1 - DOI: 10.1261/rna.125506
Coden: RNARF
Affiliations: Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria; Department of Biochemistry, Old Addenbrookes Site, University of Cambridge, 80 Tennis Court Road, United Kingdom; Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr Gasse 1, A-1030 Vienna, Austria
Adressen: Jantsch, M.F.; Department of Chromosome Biology; Max F. Perutz Laboratories; University of Vienna; Dr. Bohr Gasse 1 A-1030 Vienna, Austria; email: [email protected]
Source-File: MFPLUniWienScopus.csv
Import aus Scopus: 2-s2.0-33751099081
Importdatum: 07.12.2006 15:11:04
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
PY - 2006
Y1 - 2006
N2 - Specific RNA recognition of proteins containing the double-strand RNA-binding domain (dsRBD) is essential for several biological pathways such as ADAR-mediated adenosine deamination, localization of RNAs by Staufen, or RNA cleavage by RNAse III. Structural analysis has demonstrated the lack of base-specific interactions of dsRBDs with either a perfect RNA duplex or an RNA hairpin. We therefore asked whether in vitro selections performed in parallel with individual dsRBDs could yield RNAs that are specifically recognized by the dsRBD on which they were selected. To this end, SELEX experiments were performed using either the second dsRBD of the RNA-editing enzyme ADAR1 or the second dsRBD of Xlrbpa, a homolog of TRBP that is involved in RISC formation. Several RNA families with high binding capacities for dsRBDs were isolated from either SELEX experiment, but no discrimination of these RNAs by different dsRBDs could be detected. The selected RNAs are highly structured, and binding regions map to two neighboring stem-loops that presumably form stacked helices and are interrupted by mismatches and bulges. Despite the lack of selective binding of SELEX RNAs to individual dsRBDS, selected RNAs can efficiently interfere with RNA editing in vivo. Published by Cold Spring Harbor Laboratory Press. Copyright Œ 2006 RNA Society.
AB - Specific RNA recognition of proteins containing the double-strand RNA-binding domain (dsRBD) is essential for several biological pathways such as ADAR-mediated adenosine deamination, localization of RNAs by Staufen, or RNA cleavage by RNAse III. Structural analysis has demonstrated the lack of base-specific interactions of dsRBDs with either a perfect RNA duplex or an RNA hairpin. We therefore asked whether in vitro selections performed in parallel with individual dsRBDs could yield RNAs that are specifically recognized by the dsRBD on which they were selected. To this end, SELEX experiments were performed using either the second dsRBD of the RNA-editing enzyme ADAR1 or the second dsRBD of Xlrbpa, a homolog of TRBP that is involved in RISC formation. Several RNA families with high binding capacities for dsRBDs were isolated from either SELEX experiment, but no discrimination of these RNAs by different dsRBDs could be detected. The selected RNAs are highly structured, and binding regions map to two neighboring stem-loops that presumably form stacked helices and are interrupted by mismatches and bulges. Despite the lack of selective binding of SELEX RNAs to individual dsRBDS, selected RNAs can efficiently interfere with RNA editing in vivo. Published by Cold Spring Harbor Laboratory Press. Copyright Œ 2006 RNA Society.
M3 - Article
SN - 1355-8382
VL - 2006
SP - 1993
EP - 2004
JO - RNA
JF - RNA
IS - 11
ER -