Selective detection of alkaloids in MALDI-TOF: the introduction of a novel matrix molecule

Andreas Schinkovitz (Korresp. Autor*in), Ghislain Tsague Kenfack, Denis Seraphin, Eric Levillain, Marylène Dias, Pascal Richomme

    Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed


    The current manuscript presents 3-[5'-(methylthio)-2,2'-bithiophen-5-ylthio]propanenitrile (MT3P), as a novel matrix molecule, which facilitates the selective ionization of alkaloids in matrix-assisted laser desorption/ionization mass spectrometry. Exhibiting strong ionizing properties at low levels of laser energy, MT3P was evaluated on 55 compounds belonging to various chemical families. The observed molecular ion yields induced by MT3P were compared with those obtained by commercially available matrices such as 1,8-dihydroxy-9,10-dihydroanthracen-9-one, alpha-cyano-4-hydroxycinnamic acid, 2,2':5',2aEuro(3)-terthiophene and 2,5-dihydroxybenzoic acid. In conclusion, MT3P displayed excellent ionization properties for 23 out of 25 investigated alkaloids, while showing little to no interaction with compounds from different chemical origin. Further, in comparison to other tested matrices, MT3P generally facilitated better ionization of alkaloids. Eventually, levels of laser energy were adjusted to obtain spectra with significantly reduced matrix noise.
    Seiten (von - bis)1697-1705
    FachzeitschriftAnalytical and Bioanalytical Chemistry
    PublikationsstatusVeröffentlicht - 2012

    ÖFOS 2012

    • 301206 Pharmakologie