Structural basis for the assembly of the type V CRISPR-associated transposon complex

Michael Schmitz, Irma Querques, Seraina Oberli, Christelle Chanez, Martin Jinek

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed


CRISPR-Cas systems have been co-opted by Tn7-like transposable elements to direct RNA-guided transposition. Type V-K CRISPR-associated transposons rely on the concerted activities of the pseudonuclease Cas12k, the AAA+ ATPase TnsC, the Zn-finger protein TniQ, and the transposase TnsB. Here we present a cryo-electron microscopic structure of a target DNA-bound Cas12k-transposon recruitment complex comprised of RNA-guided Cas12k, TniQ, a polymeric TnsC filament and, unexpectedly, the ribosomal protein S15. Complex assembly, mediated by a network of interactions involving the guide RNA, TniQ, and S15, results in R-loop completion. TniQ contacts two TnsC protomers at the Cas12k-proximal filament end, likely nucleating its polymerization. Transposition activity assays corroborate our structural findings, implying that S15 is a bona fide component of the type V crRNA-guided transposon machinery. Altogether, our work uncovers key mechanistic aspects underpinning RNA-mediated assembly of CRISPR-associated transposons to guide their development as programmable tools for site-specific insertion of large DNA payloads.

Seiten (von - bis)4999-5010.e17
PublikationsstatusVeröffentlicht - 22 Dez. 2022
Extern publiziertJa

ÖFOS 2012

  • 106023 Molekularbiologie