@inbook{6c999b19044d4bee99a3d758cd4f67df,
title = "Targeted Analysis of Chromatin Events (TACE)",
abstract = "Visualization of meiotic chromatin from pollen mother cells has become an essential technique to study meiosis in the model plant Arabidopsis thaliana. Here we present an advanced cytogenetic method that combines improved immunocytology with chromosome painting, thereby generating a tool to quantitatively analyze localization of proteins to any given genomic region. Proteins involved in different processes such as DNA double-strand break formation and recombinational repair can be visualized on meiotic chromatin with the additional feature of assessing their abundance at specific chromosomal locations.",
keywords = "Arabidopsis thaliana, Chromosome painting, FISH, Immunocytology, Meiosis",
author = "Jason Sims and Changbin Chen and Peter Schl{\"o}gelhofer and Marie-Therese Kurzbauer",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-4939-9818-0\_5",
language = "English",
isbn = "978-1-4939-9817-3",
volume = "2061",
series = "Methods in Molecular Biology",
publisher = "Humana Press, Inc.",
pages = "47--58",
editor = "M{\'o}nica Pradillo and Stefan Heckmann",
booktitle = "Plant Meiosis",
}