TY - JOUR
T1 - The Arabidopsis thaliana MND1 homologue plays a key role in meiotic homologous pairing, synapsis and recombination
AU - Kerzendorfer, Claudia-Anna
AU - Vignard, J
AU - Pedrosa Harand, Andrea
AU - Siwiec, Tanja
AU - Akimcheva, Svetlana A.
AU - Jolivet, S
AU - Sablowski, R
AU - Armstrong, Sue
AU - Schweizer, Dieter
AU - Mercier, R
AU - Schlögelhofer, Peter
N1 - DOI: 10.1242/jcs.02967
Coden: JNCSA
Affiliations: Department of Chromosome Biology, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria; Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, A-1030 Vienna, Austria; Station de GeŽneŽtique et d'AmeŽlioration des Plantes, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France; Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom; School of Bioscience, University of Birmingham, Birmingham B15 2TT, United Kingdom
Adressen: Mercier, R.; Station de GeŽneŽtique et d'AmeŽlioration des Plantes; Institut National de la Recherche Agronomique 78026 Versailles Cedex, France; email: [email protected]
Source-File: MFPLUniWienScopus.csv
Import aus Scopus: 2-s2.0-33746052092
Importdatum: 07.12.2006 15:10:08
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
PY - 2006
Y1 - 2006
N2 - Mnd1 has recently been identified in yeast as a key player in meiotic recombination. Here we describe the identification and functional characterisation of the Arabidopsis homologue, AtMND1, which is essential for male and female meiosis and thus for plant fertility. Although axial elements are formed normally, sister chromatid cohesion is established and recombination initiation appears to be unaffected in mutant plants, chromosomes do not synapse. During meiotic progression, a mass of entangled chromosomes, interconnected by chromatin bridges, and severe chromosome fragmentation are observed. These defects depend on the presence of SPO11-1, a protein that initiates recombination by catalysing DNA double-strand break (DSB) formation. Furthermore, we demonstrate that the AtMND1 protein interacts with AHP2, the Arabidopsis protein closely related to budding yeast Hop2. These data demonstrate that AtMND1 plays a key role in homologous synapsis and in DSB repair during meiotic recombination.
AB - Mnd1 has recently been identified in yeast as a key player in meiotic recombination. Here we describe the identification and functional characterisation of the Arabidopsis homologue, AtMND1, which is essential for male and female meiosis and thus for plant fertility. Although axial elements are formed normally, sister chromatid cohesion is established and recombination initiation appears to be unaffected in mutant plants, chromosomes do not synapse. During meiotic progression, a mass of entangled chromosomes, interconnected by chromatin bridges, and severe chromosome fragmentation are observed. These defects depend on the presence of SPO11-1, a protein that initiates recombination by catalysing DNA double-strand break (DSB) formation. Furthermore, we demonstrate that the AtMND1 protein interacts with AHP2, the Arabidopsis protein closely related to budding yeast Hop2. These data demonstrate that AtMND1 plays a key role in homologous synapsis and in DSB repair during meiotic recombination.
M3 - Article
SN - 0021-9533
VL - 119
SP - 2486
EP - 2496
JO - Journal of Cell Science
JF - Journal of Cell Science
IS - 12
ER -