TY - JOUR
T1 - The ecological genetics of Pseudomonas syringae from kiwifruit leaves
AU - Straub, Christina
AU - Colombi, Elena
AU - Li, Li
AU - Huang, Hongwen
AU - Templeton, Matthew D.
AU - McCann, Honour C.
AU - Rainey, Paul B.
N1 - Publisher Copyright:
© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd
PY - 2018/3/9
Y1 - 2018/3/9
N2 - Interactions between commensal microbes and invading pathogens are understudied, despite their likely effects on pathogen population structure and infection processes. We describe the population structure and genetic diversity of a broad range of co-occurring Pseudomonas syringae isolated from infected and uninfected kiwifruit during an outbreak of bleeding canker disease caused by P. syringae pv. actinidiae (Psa) in New Zealand. Overall population structure was clonal and affected by ecological factors including infection status and cultivar. Most isolates are members of a new clade in phylogroup 3 (PG3a), also present on kiwifruit leaves in China and Japan. Stability of the polymorphism between pathogenic Psa and commensal P. syringae PG3a isolated from the same leaf was tested using reciprocal invasion from rare assays in vitro and in planta. P. syringae G33C (PG3a) inhibited Psa NZ54, while the presence of Psa NZ54 enhanced the growth of P. syringae G33C. This effect could not be attributed to virulence activity encoded by the Type 3 secretion system of Psa. Together our data contribute toward the development of an ecological perspective on the genetic structure of pathogen populations.
AB - Interactions between commensal microbes and invading pathogens are understudied, despite their likely effects on pathogen population structure and infection processes. We describe the population structure and genetic diversity of a broad range of co-occurring Pseudomonas syringae isolated from infected and uninfected kiwifruit during an outbreak of bleeding canker disease caused by P. syringae pv. actinidiae (Psa) in New Zealand. Overall population structure was clonal and affected by ecological factors including infection status and cultivar. Most isolates are members of a new clade in phylogroup 3 (PG3a), also present on kiwifruit leaves in China and Japan. Stability of the polymorphism between pathogenic Psa and commensal P. syringae PG3a isolated from the same leaf was tested using reciprocal invasion from rare assays in vitro and in planta. P. syringae G33C (PG3a) inhibited Psa NZ54, while the presence of Psa NZ54 enhanced the growth of P. syringae G33C. This effect could not be attributed to virulence activity encoded by the Type 3 secretion system of Psa. Together our data contribute toward the development of an ecological perspective on the genetic structure of pathogen populations.
UR - http://www.scopus.com/inward/record.url?scp=85044427194&partnerID=8YFLogxK
U2 - 10.1111/1462-2920.14092
DO - 10.1111/1462-2920.14092
M3 - Article
C2 - 29521473
AN - SCOPUS:85044427194
SN - 1462-2912
VL - 20
SP - 2066
EP - 2084
JO - Environmental Microbiology
JF - Environmental Microbiology
IS - 6
ER -