Waves of regulated protein expression and phosphorylation rewire the proteome to drive gametogenesis in budding yeast

Rahel Wettstein, Jannik Hugener, Ludovic Gillet, Yi Hernández-Armenta, Adrian Henggeler, Jingwei Xu, Julian van Gerwen, Florian Wollweber, Meret Arter, Ruedi Aebersold, Pedro Beltrao, Martin Pilhofer, Joao Matos (Korresp. Autor*in)

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

Sexually reproducing eukaryotes employ a developmentally regulated cell division program-meiosis-to generate haploid gametes from diploid germ cells. To understand how gametes arise, we generated a proteomic census encompassing the entire meiotic program of budding yeast. We found that concerted waves of protein expression and phosphorylation modify nearly all cellular pathways to support meiotic entry, meiotic progression, and gamete morphogenesis. Leveraging this comprehensive resource, we pinpointed dynamic changes in mitochondrial components and showed that phosphorylation of the FoF1-ATP synthase complex is required for efficient gametogenesis. Furthermore, using cryoET as an orthogonal approach to visualize mitochondria, we uncovered highly ordered filament arrays of Ald4ALDH2, a conserved aldehyde dehydrogenase that is highly expressed and phosphorylated during meiosis. Notably, phosphorylation-resistant mutants failed to accumulate filaments, suggesting that phosphorylation regulates context-specific Ald4ALDH2 polymerization. Overall, this proteomic census constitutes a broad resource to guide the exploration of the unique sequence of events underpinning gametogenesis.

OriginalspracheEnglisch
Seiten (von - bis)1764-1782.e8
FachzeitschriftDevelopmental Cell
Jahrgang59
Ausgabenummer13
Frühes Online-Datum18 Juni 2024
DOIs
PublikationsstatusVeröffentlicht - 8 Juli 2024

ÖFOS 2012

  • 106023 Molekularbiologie

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