TY - JOUR
T1 - Yeast nuclear envelope subdomains with distinct abilities to resist membrane expansion
AU - Campbell, Joseph L.
AU - Lorenz, Alexander
AU - Witkin, Keren L
AU - Hays, Thomas
AU - Loidl, Josef
AU - Cohen-Fix, Orna
N1 - 19.06.2008: Datenanforderung 2324 (kein Statusverlauf, kein Scopus)
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
15.01.2009: Datenanforderung 2651 (Import Sachbearbeiter)
PY - 2006
Y1 - 2006
N2 - Little is known about what dictates the round shape of the yeast Saccharomyces cerevisiae nucleus. In spo7? mutants, the nucleus is misshapen, exhibiting a single protrusion. The Spo7 protein is part of a phosphatase complex that represses phospholipid biosynthesis. Here, we report that the nuclear protrusion of spo7? mutants colocalizes with the nucleolus, whereas the nuclear compartment containing the bulk of the DNA is unaffected. Using strains in which the nucleolus is not intimately associated with the nuclear envelope, we show that the single nuclear protrusion of spo7? mutants is not a result of nucleolar expansion, but rather a property of the nuclear membrane. We found that in spo7? mutants the peripheral endoplasmic reticulum (ER) membrane was also expanded. Because the nuclear membrane and the ER are contiguous, this finding indicates that in spo7? mutants all ER membranes, with the exception of the membrane surrounding the bulk of the DNA, undergo expansion. Our results suggest that the nuclear envelope has distinct domains that differ in their ability to resist membrane expansion in response to increased phospholipid biosynthesis. We further propose that in budding yeast there is a mechanism, or structure, that restricts nuclear membrane expansion around the bulk of the DNA. Œ 2006 by The American Society for Cell Biology.
AB - Little is known about what dictates the round shape of the yeast Saccharomyces cerevisiae nucleus. In spo7? mutants, the nucleus is misshapen, exhibiting a single protrusion. The Spo7 protein is part of a phosphatase complex that represses phospholipid biosynthesis. Here, we report that the nuclear protrusion of spo7? mutants colocalizes with the nucleolus, whereas the nuclear compartment containing the bulk of the DNA is unaffected. Using strains in which the nucleolus is not intimately associated with the nuclear envelope, we show that the single nuclear protrusion of spo7? mutants is not a result of nucleolar expansion, but rather a property of the nuclear membrane. We found that in spo7? mutants the peripheral endoplasmic reticulum (ER) membrane was also expanded. Because the nuclear membrane and the ER are contiguous, this finding indicates that in spo7? mutants all ER membranes, with the exception of the membrane surrounding the bulk of the DNA, undergo expansion. Our results suggest that the nuclear envelope has distinct domains that differ in their ability to resist membrane expansion in response to increased phospholipid biosynthesis. We further propose that in budding yeast there is a mechanism, or structure, that restricts nuclear membrane expansion around the bulk of the DNA. Œ 2006 by The American Society for Cell Biology.
U2 - 10.1091/mbc.E05-09-0839
DO - 10.1091/mbc.E05-09-0839
M3 - Article
SN - 1059-1524
VL - 17
SP - 1768
EP - 1778
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
IS - 4
ER -