Yeast nuclear envelope subdomains with distinct abilities to resist membrane expansion

Joseph L. Campbell, Alexander Lorenz, Keren L Witkin, Thomas Hays, Josef Loidl, Orna Cohen-Fix

Veröffentlichungen: Beitrag in FachzeitschriftArtikelPeer Reviewed

Abstract

Little is known about what dictates the round shape of the yeast Saccharomyces cerevisiae nucleus. In spo7? mutants, the nucleus is misshapen, exhibiting a single protrusion. The Spo7 protein is part of a phosphatase complex that represses phospholipid biosynthesis. Here, we report that the nuclear protrusion of spo7? mutants colocalizes with the nucleolus, whereas the nuclear compartment containing the bulk of the DNA is unaffected. Using strains in which the nucleolus is not intimately associated with the nuclear envelope, we show that the single nuclear protrusion of spo7? mutants is not a result of nucleolar expansion, but rather a property of the nuclear membrane. We found that in spo7? mutants the peripheral endoplasmic reticulum (ER) membrane was also expanded. Because the nuclear membrane and the ER are contiguous, this finding indicates that in spo7? mutants all ER membranes, with the exception of the membrane surrounding the bulk of the DNA, undergo expansion. Our results suggest that the nuclear envelope has distinct domains that differ in their ability to resist membrane expansion in response to increased phospholipid biosynthesis. We further propose that in budding yeast there is a mechanism, or structure, that restricts nuclear membrane expansion around the bulk of the DNA. Œ 2006 by The American Society for Cell Biology.
OriginalspracheEnglisch
Seiten (von - bis)1768-1778
Seitenumfang11
FachzeitschriftMolecular Biology of the Cell
Jahrgang17
Ausgabenummer4
DOIs
PublikationsstatusVeröffentlicht - 2006

ÖFOS 2012

  • 1060 Biologie

Fingerprint

Untersuchen Sie die Forschungsthemen von „Yeast nuclear envelope subdomains with distinct abilities to resist membrane expansion“. Zusammen bilden sie einen einzigartigen Fingerprint.

Zitationsweisen