Activity: Talks and presentations › Poster presentation › Science to Science
Description
The demand for gene expression and other molecular data on developing embryos brings with it the increasing demand for three-dimensional representations of those data and the morphologies to which they apply. Micro-computed tomography (micro-CT) relatively recent extension of x-ray-based medical CT technology applied to small samples and offering resolutions of one micron or less. We have shown that hard x-ray micro-CT imaging can demonstrate soft tissue morphology of developing embryos. We are extending that work to demonstrate high-resolution 3-D imaging of proteins and mRNAs labeled with gold-conjugated molecular probes. The first test samples show the peripheral nerve morphology of post-hatching paddlefish (Polyodon), using Nanogold labeled anti-acetylated ?-tubulin immunostaining. Work in progress includes demonstrating micro-CT imaging of gene expression using a similar technique.
Period
2006
Event title
British Societies for Cell and Developmental Biology
Joint Annual Spring Meeting