A Novel Lipidomics Workflow for Improved Human Plasma Identification and Quantification Using RPLC-MSn Methods and Isotope Dilution Strategies

Evelyn Rampler; Angela Criscuolo; Martin Zeller; Yasin El Abiead; Harald Schoeny; Gerrit Hermann; Elena Sokol; Ken Cook; David A. Peake; Bernard Delanghe; Gunda Koellensperger

doi:10.1021/acs.analchem.7b05382

A Novel Lipidomics Workflow for Improved Human Plasma Identification and Quantification Using RPLC-MSn Methods and Isotope Dilution Strategies

Evelyn Rampler (Corresponding author), Angela Criscuolo, Martin Zeller, Yasin El Abiead, Harald Schoeny, Gerrit Hermann, Elena Sokol, Ken Cook, David A. Peake, Bernard Delanghe, Gunda Koellensperger

Publications: Contribution to journal › Article › Peer Reviewed

Original language	English
Pages (from-to)	6494-6501
Number of pages	8
Journal	Analytical Chemistry
Volume	90
Issue number	11
DOIs	https://doi.org/10.1021/acs.analchem.7b05382
Publication status	Published - 5 Jun 2018

Funding

This work is supported by the University of Vienna, the Faculty of Chemistry, the Vienna Metabolomics Center (VIME; http://metabolomics.univie.ac.at/) and the research platform Chemistry Meets Microbiology and the Mass Spectrometry Center (MSC) of the University of Vienna. The LILY project received additional funding from the aws PRIZE prototype funding by the Austrian BMWFW Federal Ministry. The authors thank all members of the environmental analysis (University of Vienna) group for continuous support. Especially, we acknowledge Petra Volejnik and Sophie Neumayer for laboratory support. Moreover, the collaborative work with Thermo Fisher and ISOtopic solutions is highly appreciated. Special thanks to Christian Klaas, and Tabiwang Arrey for scientific support and discussion. The MASSTR-PLAN project has received funding from the Marie Sklodowska-Curie EU Framework for Research and Innovation Horizon 2020, under Grant Agreement No. 675132.

Austrian Fields of Science 2012

104002 Analytical chemistry

Keywords

PERFORMANCE LIQUID-CHROMATOGRAPHY
IONIZATION MASS-SPECTROMETRY
YEAST PICHIA-PASTORIS
INTERLABORATORY COMPARISON EXERCISE
POLYUNSATURATED FATTY-ACIDS
LC-MS
SACCHAROMYCES-CEREVISIAE
INTERNAL STANDARD
PARTICLE-SIZE
HIGH-PRESSURE

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10.1021/acs.analchem.7b05382

Cite this

Rampler, E., Criscuolo, A., Zeller, M., El Abiead, Y., Schoeny, H., Hermann, G., Sokol, E., Cook, K., Peake, D. A., Delanghe, B., & Koellensperger, G. (2018). A Novel Lipidomics Workflow for Improved Human Plasma Identification and Quantification Using RPLC-MSn Methods and Isotope Dilution Strategies. Analytical Chemistry, 90(11), 6494-6501. https://doi.org/10.1021/acs.analchem.7b05382

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title = "A Novel Lipidomics Workflow for Improved Human Plasma Identification and Quantification Using RPLC-MSn Methods and Isotope Dilution Strategies",

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author = "Evelyn Rampler and Angela Criscuolo and Martin Zeller and \{El Abiead\}, Yasin and Harald Schoeny and Gerrit Hermann and Elena Sokol and Ken Cook and Peake, \{David A.\} and Bernard Delanghe and Gunda Koellensperger",

note = "Publisher Copyright: {\textcopyright} 2018 American Chemical Society.",

year = "2018",

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doi = "10.1021/acs.analchem.7b05382",

language = "English",

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Rampler, E, Criscuolo, A, Zeller, M, El Abiead, Y, Schoeny, H, Hermann, G, Sokol, E, Cook, K, Peake, DA, Delanghe, B & Koellensperger, G 2018, 'A Novel Lipidomics Workflow for Improved Human Plasma Identification and Quantification Using RPLC-MSn Methods and Isotope Dilution Strategies', Analytical Chemistry, vol. 90, no. 11, pp. 6494-6501. https://doi.org/10.1021/acs.analchem.7b05382

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T1 - A Novel Lipidomics Workflow for Improved Human Plasma Identification and Quantification Using RPLC-MSn Methods and Isotope Dilution Strategies

AU - Rampler, Evelyn

AU - Criscuolo, Angela

AU - Zeller, Martin

AU - El Abiead, Yasin

AU - Schoeny, Harald

AU - Hermann, Gerrit

AU - Sokol, Elena

AU - Cook, Ken

AU - Peake, David A.

AU - Delanghe, Bernard

AU - Koellensperger, Gunda

PY - 2018/6/5

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KW - IONIZATION MASS-SPECTROMETRY

KW - YEAST PICHIA-PASTORIS

KW - INTERLABORATORY COMPARISON EXERCISE

KW - POLYUNSATURATED FATTY-ACIDS

KW - LC-MS

KW - SACCHAROMYCES-CEREVISIAE

KW - INTERNAL STANDARD

KW - PARTICLE-SIZE

KW - HIGH-PRESSURE

UR - https://www.scopus.com/pages/publications/85046539543

U2 - 10.1021/acs.analchem.7b05382

DO - 10.1021/acs.analchem.7b05382

M3 - Article

SN - 0003-2700

VL - 90

SP - 6494

EP - 6501

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 11

ER -

A Novel Lipidomics Workflow for Improved Human Plasma Identification and Quantification Using RPLC-MSn Methods and Isotope Dilution Strategies

Funding

Austrian Fields of Science 2012

Keywords

Access to Document

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