A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment

Clément Bernard, Marie Locard-Paulet, Cyril Noël, Magalie Duchateau, Quentin Giai Gianetto, Bouziane Moumen, Thomas Rattei, Yann Hechard, Lars Juhl Jensen, Mariette Matondo, Ascel Samba-Louaka (Corresponding author)

Publications: Contribution to journalArticlePeer Reviewed

Abstract

Encystment is a common stress response of most protists, including free-living amoebae. Cyst formation protects the amoebae from eradication and can increase virulence of the bacteria they harbor. Here, we mapped the global molecular changes that occur in the facultatively pathogenic amoeba Acanthamoeba castellanii during the early steps of the poorly understood process of encystment. By performing transcriptomic, proteomic, and phosphoproteomic experiments during encystment, we identified more than 150,000 previously undescribed transcripts and thousands of protein sequences absent from the reference genome. These results provide molecular details to the regulation of expected biological processes, such as cell proliferation shutdown, and reveal new insights such as a rapid phospho-regulation of sites involved in cytoskeleton remodeling and translation regulation. This work constitutes the first time-resolved molecular atlas of an encysting organism and a useful resource for further investigation of amoebae encystment to allow for a better control of pathogenic amoebae.
Original languageEnglish
Article number4104
Number of pages14
JournalNature Communications
Volume13
Issue number1
DOIs
Publication statusPublished - 14 Jul 2022

Austrian Fields of Science 2012

  • 106026 Ecosystem research

Keywords

  • Acanthamoeba castellanii/microbiology
  • Amoeba/physiology
  • Bacteria
  • Proteomics
  • Virulence
  • PROTEIN
  • AUTOPHAGY
  • IDENTIFICATION
  • REGULATOR
  • ANNOTATION
  • GROWTH
  • ENCYSTATION
  • ENRICHMENT
  • DIFFERENTIATION
  • EXPRESSION

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