TY - JOUR
T1 - Analysis of phytoestrogens in foods using sol-gel enzyme columns for sample preparation
AU - Schwartz, Heidi
AU - Cichna-Markl, Margit
N1 - DOI: 10.1007/s10971-005-2364-3
Coden: JSGTE
Affiliations: Department of Analytical and Food Chemistry, University of Vienna, Wa?hringer Straße 38, A-1090 Vienna, Austria
Adressen: Cichna-Markl, M.; Department of Analytical and Food Chemistry; University of Vienna; Wa?hringer Straße 38 A-1090 Vienna, Austria; email: [email protected]
Source-File: AnalytChemScopus.csv
Import aus Scopus: 2-s2.0-24144438637
Importdatum: 12.02.2007 12:00:16
22.10.2007: Datenanforderung 1934 (Import Sachbearbeiter)
PY - 2005
Y1 - 2005
N2 - The paper describes the development and application of a new method in phytoestrogen analysis using sol-gel cellulase columns for sample preparation. Enzyme columns were produced by entrapping commercially available cellulase by slightly modifying a simple sol-gel procedure previously optimised for immobilising antibodies. Quantitative hydrolysis of both lignan and isoflavone glycosides was achieved within 5 min. Immobilisation of the enzyme enabled the elimination of enzyme-born impurities prior to analysis. Sol-gel cellulase columns could be used for at least 15 cleavage cycles over the investigated period of two months. Π2005 Springer Science + Business Media, Inc.
AB - The paper describes the development and application of a new method in phytoestrogen analysis using sol-gel cellulase columns for sample preparation. Enzyme columns were produced by entrapping commercially available cellulase by slightly modifying a simple sol-gel procedure previously optimised for immobilising antibodies. Quantitative hydrolysis of both lignan and isoflavone glycosides was achieved within 5 min. Immobilisation of the enzyme enabled the elimination of enzyme-born impurities prior to analysis. Sol-gel cellulase columns could be used for at least 15 cleavage cycles over the investigated period of two months. Π2005 Springer Science + Business Media, Inc.
U2 - 10.1007/s10971-005-2364-3
DO - 10.1007/s10971-005-2364-3
M3 - Article
SN - 0928-0707
VL - 35
SP - 211
EP - 220
JO - Journal of Sol-Gel Science and Technology
JF - Journal of Sol-Gel Science and Technology
IS - 3
ER -