Analysis of phytoestrogens in foods using sol-gel enzyme columns for sample preparation

Heidi Schwartz; Margit Cichna-Markl

doi:10.1007/s10971-005-2364-3

Analysis of phytoestrogens in foods using sol-gel enzyme columns for sample preparation

Heidi Schwartz
, Margit Cichna-Markl (Corresponding author)

Department of Analytical Chemistry

Publications: Contribution to journal › Article › Peer Reviewed

Abstract

The paper describes the development and application of a new method in phytoestrogen analysis using sol-gel cellulase columns for sample preparation. Enzyme columns were produced by entrapping commercially available cellulase by slightly modifying a simple sol-gel procedure previously optimised for immobilising antibodies. Quantitative hydrolysis of both lignan and isoflavone glycosides was achieved within 5 min. Immobilisation of the enzyme enabled the elimination of enzyme-born impurities prior to analysis. Sol-gel cellulase columns could be used for at least 15 cleavage cycles over the investigated period of two months. Œ 2005 Springer Science + Business Media, Inc.

Original language	English
Pages (from-to)	211-220
Number of pages	10
Journal	Journal of Sol-Gel Science and Technology
Volume	35
Issue number	3
DOIs	https://doi.org/10.1007/s10971-005-2364-3
Publication status	Published - 2005

Austrian Fields of Science 2012

1040 Chemistry

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10.1007/s10971-005-2364-3

Cite this

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title = "Analysis of phytoestrogens in foods using sol-gel enzyme columns for sample preparation",

abstract = "The paper describes the development and application of a new method in phytoestrogen analysis using sol-gel cellulase columns for sample preparation. Enzyme columns were produced by entrapping commercially available cellulase by slightly modifying a simple sol-gel procedure previously optimised for immobilising antibodies. Quantitative hydrolysis of both lignan and isoflavone glycosides was achieved within 5 min. Immobilisation of the enzyme enabled the elimination of enzyme-born impurities prior to analysis. Sol-gel cellulase columns could be used for at least 15 cleavage cycles over the investigated period of two months. {\OE} 2005 Springer Science + Business Media, Inc.",

author = "Heidi Schwartz and Margit Cichna-Markl",

note = "DOI: 10.1007/s10971-005-2364-3 Coden: JSGTE Affiliations: Department of Analytical and Food Chemistry, University of Vienna, Wa?hringer Stra{\ss}e 38, A-1090 Vienna, Austria Adressen: Cichna-Markl, M.; Department of Analytical and Food Chemistry; University of Vienna; Wa?hringer Stra{\ss}e 38 A-1090 Vienna, Austria; email: [email protected] Source-File: AnalytChemScopus.csv Import aus Scopus: 2-s2.0-24144438637 Importdatum: 12.02.2007 12:00:16 22.10.2007: Datenanforderung 1934 (Import Sachbearbeiter)",

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doi = "10.1007/s10971-005-2364-3",

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T1 - Analysis of phytoestrogens in foods using sol-gel enzyme columns for sample preparation

AU - Schwartz, Heidi

AU - Cichna-Markl, Margit

N1 - DOI: 10.1007/s10971-005-2364-3 Coden: JSGTE Affiliations: Department of Analytical and Food Chemistry, University of Vienna, Wa?hringer Straße 38, A-1090 Vienna, Austria Adressen: Cichna-Markl, M.; Department of Analytical and Food Chemistry; University of Vienna; Wa?hringer Straße 38 A-1090 Vienna, Austria; email: [email protected] Source-File: AnalytChemScopus.csv Import aus Scopus: 2-s2.0-24144438637 Importdatum: 12.02.2007 12:00:16 22.10.2007: Datenanforderung 1934 (Import Sachbearbeiter)

PY - 2005

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N2 - The paper describes the development and application of a new method in phytoestrogen analysis using sol-gel cellulase columns for sample preparation. Enzyme columns were produced by entrapping commercially available cellulase by slightly modifying a simple sol-gel procedure previously optimised for immobilising antibodies. Quantitative hydrolysis of both lignan and isoflavone glycosides was achieved within 5 min. Immobilisation of the enzyme enabled the elimination of enzyme-born impurities prior to analysis. Sol-gel cellulase columns could be used for at least 15 cleavage cycles over the investigated period of two months. Œ 2005 Springer Science + Business Media, Inc.

AB - The paper describes the development and application of a new method in phytoestrogen analysis using sol-gel cellulase columns for sample preparation. Enzyme columns were produced by entrapping commercially available cellulase by slightly modifying a simple sol-gel procedure previously optimised for immobilising antibodies. Quantitative hydrolysis of both lignan and isoflavone glycosides was achieved within 5 min. Immobilisation of the enzyme enabled the elimination of enzyme-born impurities prior to analysis. Sol-gel cellulase columns could be used for at least 15 cleavage cycles over the investigated period of two months. Œ 2005 Springer Science + Business Media, Inc.

U2 - 10.1007/s10971-005-2364-3

DO - 10.1007/s10971-005-2364-3

M3 - Article

SN - 0928-0707

VL - 35

SP - 211

EP - 220

JO - Journal of Sol-Gel Science and Technology

JF - Journal of Sol-Gel Science and Technology

IS - 3

ER -

Analysis of phytoestrogens in foods using sol-gel enzyme columns for sample preparation

Abstract

Austrian Fields of Science 2012

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