Ancient DNA diffuses from human bones to cave stones

Mohamed S Sarhan (Corresponding author), Achim Lehmkuhl, Rainer Straub, Adrian James Tett, Günther Wieland, Michael Franken, Albert Zink, Frank Maixner (Corresponding author)

Publications: Contribution to journalArticlePeer Reviewed

Abstract

Recent studies have demonstrated the potential to recover ancient human mitochondrial DNA and nuclear DNA from cave sediments. However, the source of such sedimentary ancient DNA is still under discussion. Here we report the case of a Bronze Age human skeleton, found in a limestone cave, which was covered with layers of calcite stone deposits. By analyzing samples representing bones and stone deposits from this cave, we were able to: i) reconstruct the full human mitochondrial genome from the bones and the stones (same haplotype); ii) determine the sex of the individual; iii) reconstruct six ancient bacterial and archaeal genomes; and finally iv) demonstrate better ancient DNA preservation in the stones than in the bones. Thereby, we demonstrate the direct diffusion of human DNA from bones into the surrounding environment and show the potential to reconstruct ancient microbial genomes from such cave deposits, which represent an additional paleoarcheological archive resource.
Original languageEnglish
Article number103397
Pages (from-to)103397
Number of pages10
JournalIscience
Volume24
Issue number12
Early online date23 Nov 2021
DOIs
Publication statusPublished - 17 Dec 2021

Austrian Fields of Science 2012

  • 106005 Bioinformatics
  • 102004 Bioinformatics
  • 106022 Microbiology

Keywords

  • biological sciences
  • evolutionary biology
  • paleobiology
  • paleogenetics
  • anthropology
  • archeology
  • Archeology
  • Evolutionary biology
  • Paleogenetics
  • Anthropology
  • Biological sciences
  • Paleobiology

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