TY - JOUR
T1 - Application of capillary electrophoresis-mass spectrometry for the investigation of the binding behavior of oxaliplatin to 5'-GMP in the presence of the sulfur-containing amino acid-L-methionine
AU - Strickmann, Dirk B.
AU - Kueng, Angelika
AU - Keppler, Bernhard
N1 - DOI: 10.1002/1522-2683(200201)23:1<74::AID-ELPS74>3.0.CO;2-F
Coden: ELCTD
Affiliations: Institute of Inorganic Chemistry, University of Vienna, Waehringerstrasse 42, A-1090 Vienna, Austria
Adressen: Keppler, B.K.; Institute of Inorganic Chemistry; University of Vienna; Waehringerstrasse 42 A-1090 Vienna, Austria; email: [email protected]
Source-File: ChemieErgScopus.csv
Import aus Scopus: 2-s2.0-0036150179
Importdatum: 09.01.2007 14:13:20
12.02.2008: Datenanforderung 2112 (Import Sachbearbeiter)
09.02.2010: Datenanforderung UNIVIS-DATEN-DAT.RA-2 (Import Sachbearbeiter)
PY - 2002
Y1 - 2002
N2 - Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) has been used for investigating the influence of the sulfur containing amino acid L-methionine (L-Met) on the binding behavior of oxaliplatin (trans-R,R-diaminocyclohexane-(oxalato) platinum(II)) to 5'-GMP. L-Methionine competes with 5'-GMP for the platinum binding site and forms as well as 5'-GMP adducts with oxaliplatin. The formation of the prognosed complexes [Pt(DACH)(L-Met-S,N)]+ and [Pt(DACH)(5'-GMP)2]2- (DACH = 1,2-diaminocyclohexane) could be proved directly by using CE-ESI-MS. Furthermore, we could now bring forward proofs, that the coordination of 5'-GMP with oxaliplatin is inhibited by L-methionine and could show, that the 5'-GMP ligands of the [Pt(DACH) (5'-GMP)2]2- complex can be replaced slowly by L-methionine whereas methionine can not be replaced by GMP.
AB - Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) has been used for investigating the influence of the sulfur containing amino acid L-methionine (L-Met) on the binding behavior of oxaliplatin (trans-R,R-diaminocyclohexane-(oxalato) platinum(II)) to 5'-GMP. L-Methionine competes with 5'-GMP for the platinum binding site and forms as well as 5'-GMP adducts with oxaliplatin. The formation of the prognosed complexes [Pt(DACH)(L-Met-S,N)]+ and [Pt(DACH)(5'-GMP)2]2- (DACH = 1,2-diaminocyclohexane) could be proved directly by using CE-ESI-MS. Furthermore, we could now bring forward proofs, that the coordination of 5'-GMP with oxaliplatin is inhibited by L-methionine and could show, that the 5'-GMP ligands of the [Pt(DACH) (5'-GMP)2]2- complex can be replaced slowly by L-methionine whereas methionine can not be replaced by GMP.
U2 - 10.1002/1522-2683(200201)23:1<74::AID-ELPS74>3.0.CO;2-F
DO - 10.1002/1522-2683(200201)23:1<74::AID-ELPS74>3.0.CO;2-F
M3 - Article
SN - 0173-0835
VL - 23
SP - 74
EP - 80
JO - Electrophoresis
JF - Electrophoresis
IS - 1
ER -