Caenorhabditis elegans as model to study natural products affecting metabolism and lifespan

T. Lehner, B. Kirchweger, J. Zwirchmayr, A. Tahir, D. Pretsch, J. M. Rollinger

Publications: Contribution to conferencePaperPeer Reviewed

Abstract

The nematode Caenorhabditis elegans was introduced as a model organism in biological research by Sydney Brenner in the 1970s. Since then, it has been increasingly used for investigating processes such as ageing, oxidative stress, neurodegeneration, or inflammation, for which there is a high degree of homology between C. elegans and human pathways, so that the worm offers promising possibilities to study mechanisms of action and effects of phytochemicals of foods and plants. In this paper, the genes and pathways regulating oxidative stress in C. elegans are discussed, as well as the methodological approaches used for their evaluation in the worm. In particular, the following aspects are reviewed: the use of stress assays, determination of chemical and biochemical markers (e.g., ROS, carbonylated proteins, lipid peroxides or altered DNA), influence on gene expression and the employment of mutant worm strains, either carrying loss-of-function mutations or fluorescent reporters, such as the GFP.
Original languageEnglish
Pages1520-1520
Number of pages1
DOIs
Publication statusPublished - Dec 2019
Event67th International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research (GA) - Innsbruck, Austria
Duration: 1 Sept 20195 Sept 2019

Conference

Conference67th International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research (GA)
Country/TerritoryAustria
CityInnsbruck
Period1/09/195/09/19

Austrian Fields of Science 2012

  • 301204 Pharmacognosy

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