Capsaicin, nonivamide and trans-pellitorine decrease free fatty acid uptake without TRPV1 activation and increase acetyl-coenzyme A synthetase activity in Caco-2 cells

Barbara Rohm (Corresponding author), Annett Riedel, Jakob P. Ley, Sabine Widder, Gerhard E. Krammer, Veronika Somoza

Publications: Contribution to journalArticlePeer Reviewed

Abstract

Red pepper and its major pungent component, capsaicin, have been associated with hypolipidemic effects in rats, although mechanistic studies on the effects of capsaicin and/or structurally related compounds on lipid metabolism are scarce. In this work, the effects of capsaicin and its structural analog nonivamide, the aliphatic alkamide trans-pellitorine and vanillin as the basic structural element of all vanilloids on the mechanisms of intestinal fatty acid uptake in differentiated intestinal Caco-2 cells were studied. Capsaicin and nonivamide were found to reduce fatty acid uptake, with IC 50 values of 0.49 μM and 1.08 μM, respectively. trans-Pellitorine was shown to reduce fatty acid uptake by 14.0 ± 2.14% at 100 μM, whereas vanillin was not effective, indicating a pivotal role of the alkyl chain with the acid amide group in fatty acid uptake by Caco-2 cells. This effect was associated neither with the activation of the transient receptor potential cation channel subfamily V member 1 (TRPV1) or the epithelial sodium channel (ENaC) nor with effects on paracellular transport or glucose uptake. However, acetyl-coenzyme A synthetase activity increased (p < 0.05) in the presence of 10 μM capsaicin, nonivamide or trans-pellitorine, pointing to an increased fatty acid biosynthesis that might counteract the decreased fatty acid uptake. This journal is

Original languageEnglish
Pages (from-to)173-185
Number of pages13
JournalFood & Function: linking the chemistry and physics of food with health and nutrition
Volume6
Issue number1
DOIs
Publication statusPublished - 2015

Austrian Fields of Science 2012

  • 106002 Biochemistry
  • 303009 Nutritional sciences

Keywords

  • LINE CACO-2
  • QUANTITATIVE PCR
  • ION-TRANSPORT
  • DELTA-SUBUNIT
  • RED-PEPPER
  • GREEN TEA
  • RATS
  • PERMEABILITY
  • CHANNELS
  • RESINIFERATOXIN

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