Abstract
Red pepper and its major pungent component, capsaicin, have been associated with hypolipidemic effects in rats, although mechanistic studies on the effects of capsaicin and/or structurally related compounds on lipid metabolism are scarce. In this work, the effects of capsaicin and its structural analog nonivamide, the aliphatic alkamide trans-pellitorine and vanillin as the basic structural element of all vanilloids on the mechanisms of intestinal fatty acid uptake in differentiated intestinal Caco-2 cells were studied. Capsaicin and nonivamide were found to reduce fatty acid uptake, with IC 50 values of 0.49 μM and 1.08 μM, respectively. trans-Pellitorine was shown to reduce fatty acid uptake by 14.0 ± 2.14% at 100 μM, whereas vanillin was not effective, indicating a pivotal role of the alkyl chain with the acid amide group in fatty acid uptake by Caco-2 cells. This effect was associated neither with the activation of the transient receptor potential cation channel subfamily V member 1 (TRPV1) or the epithelial sodium channel (ENaC) nor with effects on paracellular transport or glucose uptake. However, acetyl-coenzyme A synthetase activity increased (p < 0.05) in the presence of 10 μM capsaicin, nonivamide or trans-pellitorine, pointing to an increased fatty acid biosynthesis that might counteract the decreased fatty acid uptake. This journal is
Original language | English |
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Pages (from-to) | 173-185 |
Number of pages | 13 |
Journal | Food & Function: linking the chemistry and physics of food with health and nutrition |
Volume | 6 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2015 |
Austrian Fields of Science 2012
- 106002 Biochemistry
- 303009 Nutritional sciences
Keywords
- LINE CACO-2
- QUANTITATIVE PCR
- ION-TRANSPORT
- DELTA-SUBUNIT
- RED-PEPPER
- GREEN TEA
- RATS
- PERMEABILITY
- CHANNELS
- RESINIFERATOXIN