Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans

S. B. Zotchev; L. I. Soldatova; A. V. Orekhov; H. Schrempf

doi:10.1016/0923-2508(92)90071-U

Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans

S. B. Zotchev, L. I. Soldatova, A. V. Orekhov, H. Schrempf

Publications: Contribution to journal › Article › Peer Reviewed

Abstract

Streptomyces bambergiensis S712 harbours a giant linear plasmid PSB1 of 640 kb. After mating with the plasmidless S. lividans strain TK64, conjugants carrying a smaller extrachromosomal DNA element, pBL1, were identified. pBL1 is a 43-kb linear DNA molecule bound to a protein which protects it from attack by both 3′- and 5′-exonucleases. The absence of this protein drastically reduces the transforming efficiency of pBL1. pBL1 shares homology with linear plasmids and chromosomal DNA from S. bambergiensis strains.

Original language	English
Pages (from-to)	839-845
Number of pages	7
Journal	Research in Microbiology
Volume	143
Issue number	9
DOIs	https://doi.org/10.1016/0923-2508(92)90071-U
Publication status	Published - 1992
Externally published	Yes

Austrian Fields of Science 2012

106013 Genetics
106022 Microbiology

Keywords

DNA, Plasmid, Mating, Streptomyces bambergiensis, Streptomyces lividans
Transfer, Linear, DNA, Physical map

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10.1016/0923-2508(92)90071-U

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title = "Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans",

abstract = "Streptomyces bambergiensis S712 harbours a giant linear plasmid PSB1 of 640 kb. After mating with the plasmidless S. lividans strain TK64, conjugants carrying a smaller extrachromosomal DNA element, pBL1, were identified. pBL1 is a 43-kb linear DNA molecule bound to a protein which protects it from attack by both 3′- and 5′-exonucleases. The absence of this protein drastically reduces the transforming efficiency of pBL1. pBL1 shares homology with linear plasmids and chromosomal DNA from S. bambergiensis strains.",

keywords = "DNA, Plasmid, Mating, Streptomyces bambergiensis, Streptomyces lividans, Transfer, Linear, DNA, Physical map",

author = "Zotchev, {S. B.} and Soldatova, {L. I.} and Orekhov, {A. V.} and H. Schrempf",

year = "1992",

doi = "10.1016/0923-2508(92)90071-U",

language = "English",

volume = "143",

pages = "839--845",

journal = "Research in Microbiology",

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T1 - Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans

AU - Zotchev, S. B.

AU - Soldatova, L. I.

AU - Orekhov, A. V.

AU - Schrempf, H.

PY - 1992

Y1 - 1992

N2 - Streptomyces bambergiensis S712 harbours a giant linear plasmid PSB1 of 640 kb. After mating with the plasmidless S. lividans strain TK64, conjugants carrying a smaller extrachromosomal DNA element, pBL1, were identified. pBL1 is a 43-kb linear DNA molecule bound to a protein which protects it from attack by both 3′- and 5′-exonucleases. The absence of this protein drastically reduces the transforming efficiency of pBL1. pBL1 shares homology with linear plasmids and chromosomal DNA from S. bambergiensis strains.

AB - Streptomyces bambergiensis S712 harbours a giant linear plasmid PSB1 of 640 kb. After mating with the plasmidless S. lividans strain TK64, conjugants carrying a smaller extrachromosomal DNA element, pBL1, were identified. pBL1 is a 43-kb linear DNA molecule bound to a protein which protects it from attack by both 3′- and 5′-exonucleases. The absence of this protein drastically reduces the transforming efficiency of pBL1. pBL1 shares homology with linear plasmids and chromosomal DNA from S. bambergiensis strains.

KW - DNA, Plasmid, Mating, Streptomyces bambergiensis, Streptomyces lividans

KW - Transfer, Linear, DNA, Physical map

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AN - SCOPUS:0027096549

SN - 0923-2508

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Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans

Abstract

Austrian Fields of Science 2012

Keywords

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